FDY2004 (Medicinal herbs: Rheum palmatum, Paeonia suffruticosa Andrews, and Prunus davidiana), an herbal drug composition with an antiproliferative effect, is prepared by boiling, which is the most common herbal extraction method in traditional Korean medicine. Several parameters are considered in the process, including herb-to-solvent ratio, extraction temperature and pressure, and total decoction time. The aim of this study was to examine the physicochemical changes, index compound analysis results, antioxidant activity, and antiproliferative activity of FDY2004 according to the decoction duration to establish the conditions that ensure efficacy while minimizing side effects. Different samples of FDY2004 were obtained by decocting for 30, 60, 90, 120, 180, and 240 minutes. Each sample was evaluated for hydrogen ion concentration (pH), total soluble solid content (TSSC), index compound profiles, and antioxidative and antiproliferative activity. pH was found to decrease, while TSSC increased with an increase in decoction duration. Index compound contents for FDY2004 (aloe emodin, emodin, rhein, chrysophanol, physcion, and sennoside A for R. palmatum, paeonol for P. suffruticosa Andrews, and amygdalin for P. davidiana) increased when the decoction duration was 120 minutes or more, while the content of sennoside A did not increase. The total d-glucose amount increased with an increase in boiling duration. Antioxidant activity of FDY2004 increased when the decoction duration was 120 minutes or more, and the antiproliferative activity of FDY2004 was concentration dependent. The decoction duration for FDY2004 needs to be carefully determined so as to maintain efficacy while reducing side effects related to digestive absorption.

FDY2004（草药：大黄，牡丹安卓和山桃）是一种具有抗增殖作用的草药组合物，它是通过煮沸制备的，这是韩国传统医学中最常见的草药提取方法。在此过程中考虑了几个参数，包括草药与溶剂的比例，提取温度和压力以及总煎煮时间。这项研究的目的是根据煎药时间检查FDY2004的理化变化，指标化合物分析结果，抗氧化剂活性和抗增殖活性，以建立确保疗效同时将副作用最小化的条件。通过煎煮30、60、90、120、180和240分钟获得FDY2004的不同样品。评估每个样品的氢离子浓度（pH），总可溶性固形物含量（TSSC），指标化合物概况，以及抗氧化和抗增殖活性。发现pH降低，而TSSC随着煎煮持续时间的增加而增加。FDY2004的索引化合物含量（芦荟大黄素，大黄素，大黄酸，大黄酚，physcion和森诺甙A用于大黄，丹皮为P.牡丹安德鲁斯，苦杏仁苷和为山杨）增加时煎剂持续时间为120分钟或更多，而番泻苷A的含量并没有增加。总d葡萄糖量的增加在沸腾的持续时间增加。当煎煮时间为120分钟或更长时，FDY2004的抗氧化活性增加，并且FDY2004的抗增殖活性与浓度有关。FDY2004的煎药持续时间需要仔细确定，以便在保持功效的同时减少与消化吸收有关的副作用。