The bicyclic sesquiterpene β-caryophyllene (BCP) has diverse biological activities, including antioxidant, anti-inflammatory, antidiabetic, and analgesic effects. This study evaluates anxiolytic, toxicity, and antioxidant effects of BCP using in vitro and in vivo test models. The anxiolytic effects were tested in Swiss albino mice (Mus musculus) by applying the elevated plus-maze, rota-rod, light and dark, and hiding sphere models, while the toxicity was evaluated by brine shrimp (Artemia salina) lethality bioassay. Additionally, the antioxidant capacity was tested by using 2,2-diphenyl-1-picrylhydrazyl radical, 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid hydroxyl radical scavenging, and the Saccharomyces cerevisiae test model. The results suggest that BCP exerted a dose-dependent anxiolytic-like effect on the experimental animals. It did not show toxicity in A. salina at 24 hours. BCP showed a concentration-dependent free-radical-scavenging capacity, similar to the standard antioxidant Trolox. It also showed protective and repair capacities against hydrogen peroxide-induced damaging effects in isogenic and wild-type S. cerevisiae strains. Taken together, BCP exerted antioxidant and protective effects, which can be targeted to treat neurological diseases and disorders such as anxiety.

双环倍半萜烯β-石竹烯（BCP）具有多种生物活性，包括抗氧化，抗炎，抗糖尿病和止痛作用。这项研究使用体外和体内测试模型评估BCP的抗焦虑，毒性和抗氧化作用。抗焦虑效应在Swiss白化小鼠（测试小家鼠通过将高架十字迷宫，转棒，明暗，和隐藏球体模型），而毒性用盐水虾（评价卤虫）杀伤力生物测定。另外，通过使用2，2-二苯基-1-吡啶并肼基，2，2′-叠氮基-3-乙基苯并噻唑啉-6-磺酸羟基自由基和酿酒酵母来测试抗氧化能力。测试模型。结果表明，BCP对实验动物具有剂量依赖性的抗焦虑作用。它在24小时内对盐沼曲霉没有显示毒性。BCP显示出与浓度有关的自由基清除能力，类似于标准抗氧化剂Trolox。它还显示出对同基因和野生型酿酒酵母菌株过氧化氢诱导的破坏作用的保护和修复能力。总之，BCP发挥了抗氧化和保护作用，可用于治疗神经系统疾病和焦虑症。