Point-of-care analyzers (POCAs) for quantitative assessment of bile acids (BAs) are scarce in veterinary medicine. We evaluated the Fuji Dri-Chem Immuno AU10V analyzer and v-BA test kit (Fujifilm) for detection of feline and canine total serum BA concentration. Results were compared with a 5th-generation assay as reference method and a 3rd-generation assay, both run on a bench-top analyzer. Analytical performance was assessed at 3 different concentration ranges, and with interferences. For method comparison, samples of 60 healthy and diseased cats and 64 dogs were included. Linearity was demonstrated for a BA concentration up to 130 µmol/L in cats (r = 0.99) and 110 µmol/L in dogs (r = 0.99). The analyzer showed high precision near the lower limit of quantification of 2 µmol/L reported by the manufacturer. Intra- and inter-assay coefficients of variation were < 5% for both species and all concentrations. Interferences were observed for bilirubin (800 mg/L) and lipid (4 g/L). There was excellent correlation with the reference method for feline (r_s = 0.98) and canine samples (r_s = 0.97), with proportional biases of 6.7% and −1.3%, respectively. However, a large bias (44.1%) was noted when the POCA was compared to the 3rd-generation assay. Total observed error was less than total allowable error at the 3 concentrations. The POCA reliably detected feline and canine BA in clinically relevant concentrations.

用于定量评估胆汁酸 (BA) 的即时分析仪 (POCA) 在兽医学中很少见。我们评估了 Fuji Dri-Chem 免疫 AU10V 分析仪和 v-BA 检测试剂盒（Fujifilm）用于检测猫科动物和犬科动物血清总 BA 浓度。将结果与作为参考方法的第五代测定和第三代测定进行比较，两者均在台式分析仪上运行。在 3 个不同的浓度范围和干扰情况下评估分析性能。为了进行方法比较，纳入了 60 只健康和患病的猫以及 64 只狗的样本。猫中 BA 浓度高达 130 µmol/L ( r = 0.99) 和狗中 BA 浓度高达 110 µmol/L ( r = 0.99) 时均表现出线性。该分析仪显示出接近制造商报告的 2 µmol/L 定量下限的高精度。两个物种和所有浓度的测定内和测定间变异系数均< 5%。观察到胆红素 (800 mg/L) 和脂质 (4 g/L) 的干扰。猫科动物 ( _rs = 0.98) 和犬科动物样本 ( _rs = 0.97) 与参考方法具有极好的相关性，比例偏差分别为 6.7% 和 -1.3%。然而，当 POCA 与第三代检测进行比较时，发现存在较大偏差 (44.1%)。 3 个浓度下的总观察误差小于总允许误差。 POCA 可靠地检测出临床相关浓度的猫科动物和犬科动物 BA。