Rotaviruses (RVs) have been identified as one of the main infectious causes of diarrhea in young pigs. We determined the prevalence of rotavirus A (RVA), C (RVC), and H (RVH) in pigs on a Brazilian farm. Samples were screened by reverse-transcription (RT)-PCR, and samples positive for RVA were genotyped by PCR amplification and sequencing analysis. Of the 329 fecal samples analyzed, 102 (30.9%) were positive for RV, 25 (7.6%) contained RVA only, 32 (9.7%) contained RVC only, and 31 (9.4%) contained RVH only. Co-circulation, the presence of ≥ 2 RVs in a sample, was detected in 14 (4.2%) samples. Of the 15 animals with diarrhea, 6 (40%) were positive for RV, and of the 314 asymptomatic animals, 96 (30.6%) were positive for RV; there was no statistically significant difference between the 2 groups (p = 0.441). Genotyping of RVA strains showed co-circulation of genotypes G1, G3, G9-P[8]-I1, and I2-E1. Phylogenetic analysis showed that some of the RVA genotypes found in pigs had high percentages of identity when compared with reference strains from humans, which suggests interspecies transmission. Because RVs may be zoonotic, excretion of RVs into the environment can result in transmission to agricultural workers causing interspecies infections and allowing the emergence of new reassorted viruses.

轮状病毒 (RV) 已被确定为幼猪腹泻的主要感染原因之一。我们确定了巴西农场猪中轮状病毒 A (RVA)、C (RVC) 和 H (RVH) 的流行情况。通过逆转录 (RT)-PCR 筛选样品，通过 PCR 扩增和测序分析对 RVA 阳性的样品进行基因分型。在分析的 329 个粪便样本中，102 个 (30.9%) 为 RV 阳性，25 个 (7.6%) 仅含有 RVA，32 个 (9.7%) 仅含有 RVC，31 个 (9.4%) 仅含有 RVH。在 14 个 (4.2%) 样本中检测到共循环，即样本中存在 ≥ 2 个 RV。在 15 只腹泻动物中，6 只（40%）为 RV 阳性，在 314 只无症状动物中，96 只（30.6%）为 RV 阳性；2组间差异无统计学意义（p= 0.441）。RVA 菌株的基因分型显示基因型 G1、G3、G9-P[8]-I1 和 I2-E1 的共循环。系统发育分析表明，与来自人类的参考菌株相比，在猪身上发现的一些 RVA 基因型具有较高的同一性百分比，这表明存在种间传播。由于 RV 可能是人畜共患的，因此将 RV 排泄到环境中会导致向农业工人传播，从而引起种间感染并允许出现新的重组病毒。