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KCNQ1OT1 accelerates gastric cancer progression via miR-4319/DRAM2 axis
International Journal of Immunopathology and Pharmacology ( IF 3.0 ) Pub Date : 2020-10-25 , DOI: 10.1177/2058738420954598
Jijun Wang 1, 2 , Fan Wu 1, 2 , Yaoyao Li 3 , Lei Pang 3 , Xiaohong Wang 1, 2 , Guimei Kong 4 , Tong Zhang 5 , Duonan Yu 1, 2, 5
Affiliation  

Introduction:

This work was to explore the connection of KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) and microRNA-4319 (miR-4319), and to investigate the associated underlying mechanisms in gastric cancer (GC) progression.

Methods:

Quantitative real-time PCR was performed to measure KCNQ1OT1, miR-4319 and DNA-damage regulated autophagy modulator 2 (DRAM2) expression levels in GC cells. Moreover, expression level of KCNQ1OT1 and DRAM2 in GC tissues was analyzed at ENCORI website (http://starbase.sysu.edu.cn/index.php). Cell proliferation, colony formation assay and flow cytometry assays were performed to analyze effects of KCNQ1OT1, miR-4319 and DRAM2 on cell growth and death. Dual-luciferase activity reporter assay and RNA immunoprecipitation assay was conducted to verify the interactions of KCNQ1OT1 or DRAM2 and miR-4319.

Results and Conclusion:

We found KCNQ1OT1 level was increased in tumor tissues and cells. Force the expression of KCNQ1OT1 promotes, while knockdown KCNQ1OT1 inhibits GC cell growth. Further studies indicated miR-4319 functioned as a bridge between KCNQ1OT1 and DRAM2. Finally, we showed KCNQ1OT1/miR-4319/DRAM2 axis regulates GC cell growth in vitro and in vivo. lncRNA KCNQ1OT1 promotes GC progression by sponging miR-4319 to upregulate DRAM2, indicating KCNQ1OT1 might be a promising target for GC treatment.



中文翻译:

KCNQ1OT1 通过 miR-4319/DRAM2 轴加速胃癌进展

介绍:

这项工作旨在探索 KCNQ1 反链/反义转录物 1 (KCNQ1OT1) 和 microRNA-4319 (miR-4319) 的联系,并研究胃癌 (GC) 进展的相关潜在机制。

方法:

进行定量实时 PCR 以测量 GC 细胞中 KCNQ1OT1、miR-4319 和 DNA 损伤调节的自噬调节剂 2 (DRAM2) 的表达水平。此外,在 ENCORI 网站 (http://starbase.sysu.edu.cn/index.php) 上分析了 KCNQ1OT1 和 DRAM2 在 GC 组织中的表达水平。进行细胞增殖、集落形成测定和流式细胞术测定以分析KCNQ1OT1、miR-4319和DRAM2对细胞生长和死亡的影响。进行双荧光素酶活性报告基因测定和 RNA 免疫沉淀测定以验证 KCNQ1OT1 或 DRAM2 与 miR-4319 的相互作用。

结果和结论:

我们发现肿瘤组织和细胞中 KCNQ1OT1 水平升高。强制 KCNQ1OT1 的表达促进,而敲低 KCNQ1OT1 抑制 GC 细胞生长。进一步的研究表明 miR-4319 作为 KCNQ1OT1 和 DRAM2 之间的桥梁。最后,我们展示了 KCNQ1OT1/miR-4319/DRAM2 轴在体外和体内调节 GC 细胞生长。lncRNA KCNQ1OT1 通过海绵状 miR-4319 上调 DRAM2 促进 GC 进展,表明 KCNQ1OT1 可能是 GC 治疗的一个有希望的靶点。

更新日期:2020-10-29
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