Fluorescent Peptide Biosensor for Probing CDK6 Kinase Activity in Lung Cancer Cell Extracts,ChemBioChem

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Fluorescent Peptide Biosensor for Probing CDK6 Kinase Activity in Lung Cancer Cell Extracts
ChemBioChem ( IF 2.6 ) Pub Date : 2020-10-28 , DOI: 10.1002/cbic.202000677
Jessica Soamalala ₁ , Sebastien Diot ₁ , Morgan Pellerano ₁ , Christophe Blanquart ₂ , Mathieu Galibert ₃ , Magali Jullian ₃ , Karine Puget ₃ , May C Morris ₁

Affiliation

Distinguished kinases: We describe the design, engineering and characterisation of a fluorescent peptide biosensor derived from 6‐phosphofructokinase, that reports on CDK6 kinase activity in vitro and in lung cancer cell extracts. Implementation of this biosensor in mesothelioma cell lines derived from patients highlights differences between CDK6 kinase activity and closely related CDK4 kinase activity.

中文翻译：

用于探测肺癌细胞提取物中 CDK6 激酶活性的荧光肽生物传感器

独特的激酶：我们描述了源自 6-磷酸果糖激酶的荧光肽生物传感器的设计、工程和表征，该传感器报告了体外和肺癌细胞提取物中的 CDK6 激酶活性。这种生物传感器在源自患者的间皮瘤细胞系中的实施突出了 CDK6 激酶活性与密切相关的 CDK4 激酶活性之间的差异。

更新日期：2020-10-28

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