Circulating biological markers, such as miRNAs, hold the greatest possibilities to complement tissue biopsy and clinical diagnostic tests. The objective of this study was to evaluate the relative abundance of three circulating miRNAs in serum from 17 HPV16‐positive patients with early cervical lesions known as Low‐Grade Squamous Intraepithelial Lesions (LSILs). The expression of circulating microRNAs miR‐15b, miR‐34a and miR‐218 in patients with LSILs was compared to 23 HPV‐negative individuals showing normal cervical epithelium (healthy women) and 23 Squamous Cell Carcinoma (SCC) samples. The expression levels of miR‐15b remained unchanged while those of miRNAs 34a and 218 were relatively high in serum obtained from LSIL patients in comparison with healthy women (results were statistically significant with a p of < 0.01 or < 0.001). According to previous findings, miR‐15b was overexpressed and miRNAs 34a and 218 were underexpressed in serum from SCC patients. Additionally, the mRNA expression levels of some selected gene targets were determined [Cyclin D1 (CCND1), Cyclin E1 (CCNE1), B‐cell lymphoma 2 (Bcl‐2) and MutS homolog 2 (MSH‐2)]. All serum results correlated with tissue samples from the same patients. We propose that circulating microRNAs can be valuable as molecular markers for the early follow‐up of cervical carcinogenesis risk.

中文翻译：

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循环miR-​​15b，miR-34a和miR-218是宫颈癌发生的有希望的新型早期低侵袭性生物标志物

循环生物标记物（例如miRNA）具有最大的可能性来补充组织活检和临床诊断测试。这项研究的目的是评估17例患有早期宫颈病变的HPV16阳性患者（称为低等级鳞状上皮内病变（LSIL））的血清中三种循环miRNA的相对丰度。将LSIL患者中循环microRNA miR-15b，miR-34a和miR-218的表达与23例显示正常宫颈上皮的HPV阴性个体（健康女性）和23例鳞状细胞癌（SCC）样品进行了比较。与健康女性相比，从LSIL患者获得的血清中miR-15b的表达水平保持不变，而miRNA 34a和218的表达水平则相对较高（结果具有统计学意义，p <0.01或<0.001）。根据先前的发现，SCC患者血清中的miR-15b过表达，而miRNA 34a和218则过表达。此外，确定了某些选定基因靶标的mRNA表达水平[Cyclin D1（CCND1），Cyclin E1（CCNE1），B细胞淋巴瘤2（Bcl-2）和MutS同源物2（MSH-2）]。所有血清结果均与同一患者的组织样本相关。我们建议循环微RNA作为宫颈癌发生风险早期随访的分子标记物可能有价值。所有血清结果均与同一患者的组织样本相关。我们建议循环微RNA作为宫颈癌发生风险早期随访的分子标记物可能有价值。所有血清结果均与同一患者的组织样本相关。我们建议循环微RNA作为宫颈癌发生风险早期随访的分子标记物可能有价值。