A very effective removal of nitrate in batch and continuous experiments was achieved by a newly biofilm-formative isolated bacterium, identified by 16S rRNA as Acinetobacter EMY. The anoxic denitrifying capabilities of Acinetobacter EMY, attached to plastic biocarriers in batch and continuous moving bed bioreactors, demonstrated up to 1.75 times higher nitrate removal compared with a bacterial suspension. The denitrification rates of nitrate (200 mg/l) in the continuous operation mode were 0.39, 0.65, 1.23, and 1.14 kg-N/m3/d, with hydraulic retention times (HRTs) of 12, 8, 4, and 2 h, respectively, whereas the batch reactor removal performance showed up to 1.49 kg-N/m3/d. To the best of our knowledge, these findings are the highest values obtained for nitrate removal in comparison to previous studies focused on the characterization of denitrifying isolates. In addition, this bacterium is able to consume all of the organic matter provided in solution together with the nitrate, without leaving any residuals of organic matter in the water. This is advantageous since nitrate removal treatments by heterotrophic bacteria usually require addition of organic matter to the system, leading to secondary pollution. The isolated bacterium therefore provides a good solution for biological treatment of nitrogen in water, particularly in treatment systems that integrate immobilized biomass in the treatment process.

中文翻译：

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一种新的不动杆菌分离物是一种极其有效的生物膜形成反硝化细菌

在分批和连续实验中非常有效地去除硝酸盐是通过一种新的生物膜形成分离细菌实现的，该细菌通过 16S rRNA 鉴定为不动杆菌 EMY。EMY 不动杆菌的缺氧反硝化能力，在分批和连续移动床生物反应器中附着在塑料生物载体上，与细菌悬浮液相比，硝酸盐去除率高出 1.75 倍。连续运行模式下硝酸盐（200 mg/l）的反硝化率为0.39、0.65、1.23和1.14 kg-N/m3/d，水力停留时间（HRTs）分别为12、8、4和2 h ，而间歇式反应器的去除性能高达 1.49 kg-N/m3/d。据我们所知，与之前专注于反硝化分离物特征的研究相比，这些发现是硝酸盐去除获得的最高值。此外，这种细菌能够与硝酸盐一起消耗溶液中提供的所有有机物质，而不会在水中留下任何有机物质残留物。这是有利的，因为异养细菌的硝酸盐去除处理通常需要向系统添加有机物，从而导致二次污染。因此，分离的细菌为水中氮的生物处理提供了良好的解决方案，特别是在将固定生物质整合到处理过程中的处理系统中。不会在水中留下任何有机物残留。这是有利的，因为异养细菌的硝酸盐去除处理通常需要向系统添加有机物，从而导致二次污染。因此，分离的细菌为水中氮的生物处理提供了良好的解决方案，特别是在将固定生物质整合到处理过程中的处理系统中。不会在水中留下任何有机物残留。这是有利的，因为异养细菌的硝酸盐去除处理通常需要向系统添加有机物，从而导致二次污染。因此，分离的细菌为水中氮的生物处理提供了良好的解决方案，特别是在将固定生物质整合到处理过程中的处理系统中。