Various adjuvant effects on the immunogenicity of the candidate inactivated Puumala virus vaccine were detected in BALB/c mice. Adjuvants under study were: aluminum hydroxide, spherical particles of Tobacco mosaic virus coat protein, B subunit of heat-labile enterotoxin of Escherichia coli, and low endotoxic lipopolysaccharide of Shigella sonnei. Aluminum hydroxide (1 mg/ml) did not affect neutralizing antibodies’ induction and vaccine stability during storage compared to immunization with the vaccine without adjuvant. B subunit of heat-labile enterotoxin (0.2 µg/ml), low endotoxic lipopolysaccharide (50 µg/ml), and plant virus-based spherical particles (300 µg/ml) significantly enhance the humoral immune response of vaccine (p < 0.0001). Pronounced stimulation of IL-12 and IFN-ɣ was observed when mice were immunized with vaccines both with adjuvants (except of aluminum hydroxide) and without adjuvants. It has been shown that low endotoxic lipopolysaccharide contributes not only to enhance the immune response but also to stabilize vaccine immunogenicity during at least 1 year storage.

在BALB / c小鼠中检测到了对候选灭活的Puumala病毒疫苗免疫原性的各种佐剂作用。研究的佐剂为：氢氧化铝，烟草花叶病毒外壳蛋白的球形颗粒，热不稳定性肠毒素的B亚基大肠杆菌和低内毒素的脂多糖 志贺氏菌。与不使用佐剂的疫苗免疫相比，氢氧化铝（1 mg / ml）在存储过程中不影响中和抗体的诱导和疫苗稳定性。热不稳定肠毒素的B亚基（0.2 µg / ml），低内毒素脂多糖（50 µg / ml）和植物病毒基球形颗粒（300 µg / ml）显着增强了疫苗的体液免疫反应（p <0.0001） 。明显刺激IL-12和IFN-ɣ当同时用佐剂（氢氧化铝除外）和不加佐剂的疫苗免疫小鼠时，观察到了这种现象。已经表明，低内毒素脂多糖不仅在至少一年的储存过程中有助于增强免疫应答，而且还可以稳定疫苗的免疫原性。