Abstract Melanoma is an aggressive malignant tumor. The crucial role of circular RNAs has been documented in many types of cancer, including melanoma. The objective of this study was to uncover the function of circ_0084043 in the biological process of melanoma and associated mechanism of action. The expression of circ_0084043, miR-31, and Krüppel-like factor 3 (KLF3) was determined by qRT-PCR. Cell proliferation and apoptosis were monitored by the MTT assay and flow cytometry assay, respectively. The progression of glycolysis was evaluated according to the levels of glucose consumption, lactate production, and ATP concentration using appropriate detection kits. The relationship between miR-31 and circ_0084043 or KLF3 was predicted by the bioinformatics tool and ascertained by the dual-luciferase reporter assay. The protein levels of KLF3 and glucose transporter 1 (Glut1) were quantified by western blot. A xenograft model was established to ascertain the role of circ_0084043 in vivo. As a result, circ_0084043 expression was reinforced in melanoma tissues and cells. Circ_0084043 knockdown inhibited cell proliferation, induced cell apoptosis, and restrained glycolysis. MiR-31 was a target of circ_0084043, and miR-31 deficiency reversed the role of circ_0084043 knockdown. KLF3 was targeted by miR-31, and KLF3 upregulation abolished the effects of miR-31 enrichment. Moreover, circ_0084043 knockdown impeded tumor growth in vivo and suppressed the level of Glut1 by modulating miR-31 and KLF3. Circ_0084043 promoted cell proliferation and glycolysis, and blocked apoptosis through the circ_0084043–miR-31–KLF3 regulatory axis in melanoma.

中文翻译：

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Circ_0084043通过circ_0084043-miR-31-KLF3轴促进细胞增殖和糖酵解但阻止黑色素瘤中的细胞凋亡

摘要 黑色素瘤是一种侵袭性恶性肿瘤。环状 RNA 在包括黑色素瘤在内的多种癌症中发挥着重要作用。本研究的目的是揭示 circ_0084043 在黑色素瘤的生物学过程中的功能和相关的作用机制。通过 qRT-PCR 确定 circ_0084043、miR-31 和 Krüppel 样因子 3（KLF3）的表达。分别通过MTT测定和流式细胞术测定监测细胞增殖和凋亡。使用合适的检测试剂盒根据葡萄糖消耗、乳酸产生和 ATP 浓度的水平评估糖酵解的进展。miR-31 与 circ_0084043 或 KLF3 之间的关系由生物信息学工具预测，并由双荧光素酶报告基因测定确定。KLF3 和葡萄糖转运蛋白 1 (Glut1) 的蛋白质水平通过蛋白质印迹定量。建立异种移植模型以确定circ_0084043在体内的作用。结果，circ_0084043 表达在黑色素瘤组织和细胞中得到加强。Circ_0084043敲低抑制细胞增殖，诱导细胞凋亡，抑制糖酵解。MiR-31 是 circ_0084043 的靶标，而 miR-31 缺陷逆转了 circ_0084043 敲低的作用。KLF3 被 miR-31 靶向，KLF3 上调消除了 miR-31 富集的影响。此外，circ_0084043 敲低阻碍了体内肿瘤的生长，并通过调节 miR-31 和 KLF3 抑制了 Glut1 的水平。Circ_0084043通过黑色素瘤中的circ_0084043-miR-31-KLF3调节轴促进细胞增殖和糖酵解，并阻断细胞凋亡。