Abstract Introduction Early detection of pregnancy is vital for appropriate reproductive management programmes to facilitate the rapid re-insemination of non-pregnant females and reduce the calving interval. Material and Methods A barium chloride test was compared with a commercial progesterone ELISA to detect pregnancy in non-descriptive cows and investigate if it could be applied as an alternative to ELISA in the field. Blood and urine samples were collected from 74 cows with recorded insemination dates. The progesterone ELISA and barium chloride assay were implemented to detect progesterone (P4) in blood and urine specimens, respectively. The cows' reproductive systems were examined after they were slaughtered to determine the uterus's status. Macroscopic examination of the uterus was used as a reference standard for both tests. Results The sensitivity rates of the P4 ELISA and barium chloride test to detect pregnant cows were 100.0% and 79.4%, and to detect the corpus luteum (CL) were 83.0% and 87.0%, respectively, their sensitivity increasing in the presence of the CL. The ELISA and barium chloride tests were 79.7% and 52.7% accurate in the diagnosis of pregnancy. The accuracy of the barium chloride test in CL detection increased to 81.0%, and that of the ELISA to 86.4%. There were no significant differences (P = 0.052) between the barium chloride assay and ELISA when they were utilised for the identification of the CL. Conclusion The barium chloride test can be an inexpensive and time-saving alternative to ELISA in pregnancy diagnosis when the insemination date is known.

中文翻译：

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氯化钡试验与 ELISA 在奶牛妊娠检测中的比较

摘要 简介 早期发现妊娠对于适当的生殖管理计划至关重要，以促进未怀孕雌性的快速再授精和缩短产犊间隔。材料和方法 将氯化钡测试与商业孕酮 ELISA 进行比较，以检测非描述性奶牛的妊娠情况，并研究它是否可以在现场用作 ELISA 的替代方法。从记录授精日期的 74 头奶牛身上采集血液和尿液样本。采用孕酮 ELISA 和氯化钡试验分别检测血液和尿液样本中的孕酮 (P4)。在屠宰后检查母牛的生殖系统以确定子宫的状态。子宫的宏观检查被用作两种测试的参考标准。结果P4 ELISA法和氯化钡法检测妊娠母牛的灵敏度分别为100.0%和79.4%，检测黄体（CL）的灵敏度分别为83.0%和87.0%，在CL的存在下灵敏度增加。 . ELISA 和氯化钡测试对妊娠诊断的准确率为 79.7% 和 52.7%。CL检测氯化钡试验准确率提高到81.0%，ELISA准确率提高到86.4%。氯化钡测定和ELISA在用于鉴定CL时没有显着差异（P = 0.052）。结论 在已知授精日期的情况下，氯化钡检测可作为 ELISA 的一种廉价且省时的妊娠诊断替代方法。0%和79.4%，而对黄体（CL）的检测分别为83.0%和87.0%，它们的灵敏度在CL的存在下增加。ELISA 和氯化钡测试对妊娠诊断的准确率为 79.7% 和 52.7%。CL检测氯化钡试验准确率提高到81.0%，ELISA准确率提高到86.4%。氯化钡测定和ELISA在用于鉴定CL时没有显着差异（P = 0.052）。结论 在已知授精日期的情况下，氯化钡检测可作为 ELISA 的一种廉价且省时的妊娠诊断替代方法。0%和79.4%，而对黄体（CL）的检测分别为83.0%和87.0%，它们的灵敏度在CL的存在下增加。ELISA 和氯化钡测试对妊娠诊断的准确率为 79.7% 和 52.7%。CL检测氯化钡试验准确率提高到81.0%，ELISA准确率提高到86.4%。氯化钡测定和ELISA在用于鉴定CL时没有显着差异（P = 0.052）。结论 在已知授精日期的情况下，氯化钡检测可作为 ELISA 的一种廉价且省时的妊娠诊断替代方法。妊娠诊断准确率为 7%。CL检测氯化钡试验准确率提高到81.0%，ELISA准确率提高到86.4%。氯化钡测定和ELISA在用于鉴定CL时没有显着差异（P = 0.052）。结论 在已知授精日期的情况下，氯化钡检测可作为 ELISA 的一种廉价且省时的妊娠诊断替代方法。妊娠诊断准确率为 7%。CL检测氯化钡试验准确率提高到81.0%，ELISA准确率提高到86.4%。氯化钡测定和ELISA在用于鉴定CL时没有显着差异（P = 0.052）。结论 在已知授精日期的情况下，氯化钡检测可作为 ELISA 的一种廉价且省时的妊娠诊断替代方法。