An extract of Hypericum scruglii, an endangered endemic plant of Sardinia (Italy), was prepared and characterized. It was loaded in special phospholipid vesicles, glycerosomes, which were modified by adding maltodextrin (glucidex) and a polymer (gelatin or hyaluronan). The corresponding liposomes were also prepared and used as reference. The vesicles disclosed suitable physicochemical features for skin delivery. Indeed, their mean diameter ranged from 120 to 160 nm, they were homogeneously dispersed (polydispersity index ≤ 0.30), and their zeta potential was highly negative (~−45 mV). The vesicle dispersions maintained unchanged characteristics during 60 days of storage, were highly biocompatible, and were able to protect keratinocytes against damages due to oxidative stress induced by treating them with hydrogen peroxide. Vesicles were also capable of promoting cell proliferation and migration in vitro by means of a scratch wound assay. The results confirmed the fruitful delivery of the extract of H. scruglii in glycerosomes modified with glucidex and gelatin and their promising ability for skin protection and treatment.

中文翻译：

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贯叶连翘提取物的提取，表征和掺入专为治疗与氧化应激有关的皮肤疾病而设计的磷脂囊泡中

贯叶连翘提取物，准备并鉴定了撒丁岛（意大利）濒临灭绝的特有植物。它被装载在特殊的磷脂囊泡，甘油脂质体中，通过添加麦芽糊精（葡糖苷）和聚合物（明胶或透明质酸）进行修饰。还制备了相应的脂质体，并用作参考。囊泡公开了适合皮肤递送的理化特征。实际上，它们的平均直径在120到160 nm之间，它们被均匀分散（多分散指数≤0.30），并且它们的Zeta电位非常负（〜-45 mV）。囊泡分散液在储存60天期间保持不变的特性，具有高度的生物相容性，并且能够保护角质形成细胞免受因用过氧化氢处理而引起的氧化应激引起的损害。囊泡还能够通过刮伤试验在体外促进细胞增殖和迁移。结果证实了提取物的丰硕成果糖精和明胶修饰的甘油脂质体中的H. scruglii及其有希望的皮肤保护和治疗能力。