Significance: Axially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed. Aim: A high-speed PA with an update rate faster than the camera row read time is used to track the rolling shutter at camera-limited rates. Approach: The point spread function is evaluated to ensure sub-micron isotropic resolution, and the technique is demonstrated on a live Drosophila embryo. Results: Isotropic resolution is shown down to 720 ± 55 nm in all three spatial dimensions. With an update rate of 2.85 μs, the PA tracks the camera sensor rolling shutter at camera-limited rates. Features in the Drosophila embryo are resolved clearly compared with the equivalent static light sheet case. The random-access nature of the PA enables a camera sensor readout in the same direction for each frame to maintain even temporal sampling in image sequences with no speed loss. Conclusions: Use of PAs is compatible with axially swept light sheet microscopy and offers significant improvements in speed.

中文翻译：

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使用线性 MEMS 相控阵实现各向同性分辨率的高速轴向扫描光片显微镜

意义：轴向扫描光片显微镜用于无反卷积、高分辨率 3D 成像，但通常轴向扫描机制会降低最高成像速度。用于轴向扫描的相控阵 (PA) 可实现高分辨率和高速。目标：使用更新速率快于相机行读取时间的高速 PA 来以相机限制的速率跟踪卷帘快门。方法：评估点扩散函数以确保亚微米各向同性分辨率，并在活果蝇胚胎上演示该技术。结果：在所有三个空间维度上显示各向同性分辨率低至 720 ± 55 nm。PA 的更新率为 2.85 μs，以相机限制的速率跟踪相机传感器滚动快门。与等效的静态光片情况相比，果蝇胚胎的特征得到了清晰的解析。PA 的随机访问特性使相机传感器能够在同一方向上对每一帧进行读出，从而在图像序列中保持均匀的时间采样，而不会造成速度损失。结论：PA 的使用与轴向扫描光片显微镜兼容，并且显着提高了速度。