Abstract

“Sitopaladi churna,” a well-known formulation of Ayurveda, is prescribed to treat the disease like Bronchitis, Pneumonia, allergic conditions, viral infection of the respiratory tract and as a natural antioxidant. A novel method based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector was established and validated for sitopaladi churna. Here, HPLC fingerprints data obtained for 28 samples including standard sample were then treated to chemometric analysis like principal component analysis and hierarchical clustering analysis for further analysis to evaluate the differences in market samples of sitopaladi churna. Additionally, one major marker compound, piperine was quantified and it also facilitated for relative retention time. The simulative mean spectrum was also generated. The validation results showed that the developed method was simple, precise and stable. Thus, the developed chromatographic method adjoined with multivariate analysis can be used as an efficient and practical approach for quality assessment of sitopaladi churna.

中文翻译：

---

高效液相色谱法与多变量分析相结合对Sitopaladi Churna的质量评估

摘要

阿育吠陀的著名配方“ Sitopaladi churna”被指定用于治疗支气管炎，肺炎，过敏性疾病，呼吸道病毒感染等疾病，并作为天然抗氧化剂。建立了一种基于反相高效液相色谱（RP-HPLC）结合光电二极管阵列检测器的新方法，并验证了西他帕拉迪片的有效性。在这里，然后对包括标准样品在内的28个样品获得的HPLC指纹图谱数据进行化学计量分析（例如主成分分析和层次聚类分析），以进行进一步分析，以评估西图帕拉迪churna市场样品的差异。此外，对一种主要的标记化合物胡椒碱进行了定量，它也有助于相对保留时间。还生成了模拟平均光谱。验证结果表明，该方法简单，准确，稳定。因此，与多变量分析相结合的发达的色谱方法可以用作一种高效，实用的西帕塔拉迪栗的质量评估方法。