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Taguchi optimization and scale up of xylanase from Bacillus licheniformis isolated from hot water geyser
Journal of Genetic Engineering and Biotechnology ( IF 3.6 ) Pub Date : 2020-10-22 , DOI: 10.1186/s43141-020-00084-0
Girisha Malhotra 1 , Shilpa S Chapadgaonkar 1
Affiliation  

Xylanase is one of the widely applied industrial enzymes with diverse applications. Thermostability and alkali tolerance are the two most desirable qualities for industrial applications of xylanase. In this paper, we reveal the statistical Taguchi optimization strategy for maximization of xylanase production. The important process parameters pH, temperature, concentration of wheat bran, and concentration of yeast extract were optimized using the Taguchi L8 orthogonal array where the 4 factors were considered at 2 levels (high and low). The optimized conditions given by model were obtained as follows: (i) pH 6, (ii) culture temperature 35 °C, (iii) concentration of xylan 2% w/v, (iv) concentration of wheat bran 2.5% w/v. The production was scaled upto 2.5 L bioreactor using optimized process parameters. A high xylanase titer of 400 U/ml could be achieved in less than 60 h of culture in the reactor. Optimization was successful in achieving about threefold increase in the yield of xylanase. The optimized conditions resulted in a successful scale up and enhancement of xylanase production.

中文翻译:


热水间歇泉中地衣芽孢杆菌木聚糖酶的田口优化和放大



木聚糖酶是应用广泛的工业酶之一,用途广泛。热稳定性和耐碱性是木聚糖酶工业应用的两个最理想的品质。在本文中,我们揭示了最大化木聚糖酶产量的统计田口优化策略。使用田口 L8 正交阵列对重要的工艺参数 pH、温度、麦麸浓度和酵母提取物浓度进行优化,其中 4 个因素在 2 个水平(高和低)下考虑。模型给出的优化条件如下:(i) pH 6,(ii) 培养温度 35 °C,(iii) 木聚糖浓度 2% w/v,(iv) 麦麸浓度 2.5% w/v 。使用优化的工艺参数,生产规模扩大至 2.5 L 生物反应器。在反应器中培养不到 60 小时即可实现 400 U/ml 的高木聚糖酶效价。优化成功地将木聚糖酶的产量提高了约三倍。优化的条件成功地扩大了木聚糖酶的生产规模并提高了产量。
更新日期:2020-10-27
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