Behçet’s disease (BD) is a systemic vasculitis disorder with multifactorial immunopathogenesis and associated with significant morbidity and mortality. MicroRNAs (miRNAs) are involved in the pathogenesis of inflammatory diseases. MiR-146 and miR-155 are known key regulators of immune response. This study was conducted to determine the expression of miRNA-146a and miRNA-155 in patients with BD and to link their possible association with the clinical manifestations and activity of this disease to evaluate their role as diagnostic or prognostic markers. A total of 60 patients with BD and 25 age- and gender-matched healthy controls were examined in a case-control study from October 2017 to September 2018 for the expression levels of miR-146a and miR-155 using singleplexTaqMan two-step stem loop quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR). Patients with BD had significantly lower miR-146a levels than control subjects (P < 0.001). Regarding the miR-155 expression level, no statistically significant differences were detected between patients and healthy controls (P = 0.736). The expression level of miR-146a showed no significant association with the different clinical manifestations of patients with BD. This study suggests the possibility that miR-146a expression in patients with BD is involved in the pathogenesis of disease. Furthermore, it can be used as a diagnostic biomarker and a therapeutic target for BD in the future.

中文翻译：

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miR-146a和miR-155在埃及白塞病患者中的表达：临床意义及与疾病活动度的关系

白塞病 (BD) 是一种全身性血管炎疾病，具有多因素免疫发病机制，并与显着的发病率和死亡率相关。MicroRNAs (miRNAs) 参与炎症性疾病的发病机制。MiR-146 和 miR-155 是已知的免疫反应的关键调节剂。本研究旨在确定 miRNA-146a 和 miRNA-155 在 BD 患者中的表达，并将它们可能与该疾病的临床表现和活动联系起来，以评估它们作为诊断或预后标志物的作用。在 2017 年 10 月至 2018 年 9 月的病例对照研究中，共有 60 名 BD 患者和 25 名年龄和性别匹配的健康对照组使用 singleplexTaqMan 两步茎环检测 miR-146a 和 miR-155 的表达水平定量逆转录实时聚合酶链反应 (qRT-PCR)。BD 患者的 miR-146a 水平显着低于对照组（P < 0.001）。关于 miR-155 表达水平，患者和健康对照之间没有检测到统计学上的显着差异（P = 0.736）。miR-146a的表达水平与BD患者的不同临床表现无显着相关性。该研究提示 BD 患者的 miR-146a 表达可能参与了疾病的发病机制。此外，