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Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia
BMC Genetics ( IF 2.9 ) Pub Date : 2020-10-22 , DOI: 10.1186/s12863-020-00874-y
Natalia Blazhko , Sultan Vyshegurov , Alexander Donchenko , Kirill Shatokhin , Valeria Ryabinina , Kirill Plotnikov , Alevtina Khodakova , Sergey Pashkovskiy

This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected animals. The researchers focused on exploring the polymorphism of the env gene and, in doing so, discovered the new genotypes Ia and Ib, which differ from genotype I. Several hypotheses on the origin of the different genotypes in Siberia are discussed. We obtained varying length of the restriction fragments for genotypes I. Additionally using restrictase Hae III were received fragments was named genotype Ia, and genotype Ib. There are 2.57 ± 0.55% (20 out of 779) samples of genotype Ib which does not differ significantly from 1% (χ2 = 2.46). Other genotypes were observed in the cattle of Siberia as wild type genotypes (their frequency varied from 17.84 to 32.73%). The maximum viral load was observed in animals with the II and IV viral genotypes (1000–1400 viral particles per 1000 healthy cells), and the minimum viral load was observed animals with genotype Ib (from 700 to 900 viral particles per 1000 healthy cells). The probability of the direct introduction of genotype II from South America to Siberia is extremely small and it is more likely that the strain originated independently in an autonomous population with its distribution also occurring independently. A new variety of genotype I (Ib) was found, which can be both a neoplasm and a relict strain.

中文翻译:

西伯利亚西部牛白血病病毒env基因的基因型多样性

这项研究描述了西伯利亚西部牛白血病病毒的生物多样性和特性。本文探讨了牛白血病病毒env基因的不同基因型对感染动物血液学参数的影响。研究人员专注于探索env基因的多态性,并在此过程中发现了与基因型I不同的新基因型Ia和Ib。讨论了关于西伯利亚不同基因型起源的几种假设。我们获得了不同长度的基因型I限制性片段。另外,使用限制性酶Hae III收到的片段分别称为基因型Ia和基因型Ib。基因型Ib有2.57±0.55%(779个中的20个)样本,与1%(χ2= 2.46)没有显着差异。在西伯利亚牛中观察到其他基因型为野生型基因型(其频率从17.84%到32.73%不等)。在具有II和IV型病毒基因型的动物中观察到最大病毒载量(每1000个健康细胞1000-1400个病毒颗粒),而在具有Ib基因型的动物中观察到最小病毒载量(每1000个健康细胞700至900个病毒颗粒) 。将基因型II从南美直接引入西伯利亚的可能性非常小,而且该菌株更有可能独立于一个自治种群而又独立分布。发现了新的基因型I(Ib),既可以是肿瘤,也可以是遗留株。在具有II和IV型病毒基因型的动物中观察到最大病毒载量(每1000个健康细胞1000-1400个病毒颗粒),而在具有Ib基因型的动物中观察到最小病毒载量(每1000个健康细胞700至900个病毒颗粒) 。将基因型II从南美直接引入西伯利亚的可能性非常小,而且该菌株更有可能独立于一个自治种群而又独立分布。发现了新的基因型I(Ib),既可以是肿瘤,也可以是遗留株。在具有II和IV型病毒基因型的动物中观察到最大病毒载量(每1000个健康细胞1000-1400个病毒颗粒),而在具有Ib基因型的动物中观察到最小病毒载量(每1000个健康细胞700至900个病毒颗粒) 。将基因型II从南美直接引入西伯利亚的可能性非常小,而且该菌株更有可能独立于一个自治种群而又独立分布。发现了新的基因型I(Ib),既可以是肿瘤,也可以是遗留株。将基因型II从南美直接引入西伯利亚的可能性非常小,而且该菌株更有可能独立于一个自治种群而又独立分布。发现了新的基因型I(Ib),既可以是肿瘤,也可以是遗留株。将基因型II从南美直接引入西伯利亚的可能性非常小,而且该菌株更有可能独立于一个自治种群而又独立分布。发现了新的基因型I(Ib),既可以是肿瘤,也可以是遗留株。
更新日期:2020-10-26
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