Polylactic acid (PLA) is a thermoplastic and biodegradable polyester, largely derived from renewable resources such as corn starch, cassava starch and sugarcane. However, PLA is only soluble in a narrow range of solvents such as tetrahydrofuran, dioxane, chlorinated solvents and heated benzene. The limited choices of solvent for PLA dissolution have imposed significant challenges in the development of specifically engineered PLA nanofibers with electrospinning techniques. Generally, the electrospun polymeric materials have been rendered with unique properties such as high porosity and complex geometry while maintaining its biodegradability and biocompatibility for emerging biomedical applications. In this study, a new anticancer drug delivery system composed of PLA nanofibers with encapsulated paclitaxel was developed by the electrospinning of the respective nanofibers on top of a spin-coated thin film with the same chemical compositions. Our unique approach is meant for promoting strong bonding between PLA-based nanofibers and their respective films in order to improve the prolonged release properties and composite film stability within a fluctuative physiochemical environment during cell culture. PLA/paclitaxel nanofiber supported on respective polymeric films were probed by scanning electronic microscope, Fourier transform infrared spectrometer and water contact measurement for determining their surface morphologies, fibers’ diameters, molecular vibrational modes, and wettability, respectively. Moreover, PLA/paclitaxel nanofibers supported on respective spin-coated films at different loadings of paclitaxel were evaluated for their abilities in killing human colorectal carcinoma cells (HCT-116). More importantly, MTT assays showed that regardless of the concentrations of paclitaxel, the growth of HCT-116 was effectively inhibited by the prolonged release of paclitaxel from PLA/paclitaxel nanofibers. An effective prolonged delivery system of paclitaxel based on PLA nanofiber-based film has demonstrated exciting potentials for emerging applications as implantable drug delivery patch in post-surgical cancer eradication.

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通过静电纺丝制备用于癌症治疗的聚乳酸/紫杉醇纳米纤维

聚乳酸（PLA）是一种可生物降解的热塑性聚酯，主要来自可再生资源，例如玉米淀粉，木薯淀粉和甘蔗。但是，PLA仅溶于少量溶剂，例如四氢呋喃，二恶烷，氯化溶剂和加热的苯。用于PLA溶解的溶剂的选择有限，对采用静电纺丝技术专门设计的PLA纳米纤维的开发提出了重大挑战。通常，电纺聚合物材料已经赋予其独特的性能，例如高孔隙率和复杂的几何形状，同时保持了其对于新兴生物医学应用的生物降解性和生物相容性。在这个研究中，通过将各自的纳米纤维在具有相同化学组成的旋涂薄膜上进行静电纺丝，开发了一种由PLA纳米纤维与紫杉醇包封的新型抗癌药物递送系统。我们独特的方法旨在促进PLA基纳米纤维与它们各自的薄膜之间的牢固结合，从而改善细胞培养过程中在波动的物理化学环境中的延长释放性能和复合膜的稳定性。通过扫描电子显微镜，傅立叶变换红外光谱仪和水接触测量法分别研究了分别负载在聚合物膜上的PLA /紫杉醇纳米纤维的表面形态，纤维直径，分子振动模式和润湿性。此外，评价了分别负载在紫杉醇上不同旋涂膜上的PLA /紫杉醇纳米纤维杀死人结肠直肠癌细胞（HCT-116）的能力。更重要的是，MTT分析表明，不管紫杉醇的浓度如何，紫杉醇从PLA /紫杉醇纳米纤维中的延长释放都能有效地抑制HCT-116的生长。基于PLA纳米纤维基薄膜的紫杉醇有效的延长递送系统已显示出令人振奋的潜力，可作为新兴的应用，作为根除术后癌症的可植入药物递送贴剂。紫杉醇从PLA /紫杉醇纳米纤维中的延长释放可有效抑制HCT-116的生长。基于PLA纳米纤维基薄膜的紫杉醇有效的延长递送系统已显示出令人振奋的潜力，可作为新兴的应用，作为根除术后癌症的可植入药物递送贴剂。紫杉醇从PLA /紫杉醇纳米纤维中的延长释放可有效抑制HCT-116的生长。基于PLA纳米纤维基薄膜的紫杉醇有效的延长递送系统已显示出令人振奋的潜力，可作为新兴的应用，作为根除术后癌症的可植入药物递送贴剂。