Although OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer, its role and underlying mechanism in ovarian carcinoma (OC) remains unknown. This study aimed to investigate the role of OIP5-AS1 in OC. OC tissues and non-tumor tissues (ovary tissues within 3 cm around tumors) were collected from 58 OC patients (age range 36 to 67 years old, mean age 51.4 ± 5.9 years old). The expression of OIP5-AS1 and snail in paired tissues were determined by RT-qPCR. The interaction between OIP5-AS1 and miR-34a was predicted by IntaRNA2.0 and confirmed by dual luciferase reporter assay. The effects of overexpression of OIP5-AS1 and miR-34a on the expression of snail were analyzed by RT-qPCR and Western blotting. Cell invasion and migration were analyzed by Transwell assay. We observed that the expression of OIP5-AS1 and snail was upregulated and positively correlated with each other in OC. RNA–RNA interaction analysis showed that OIP5-AS1 might sponge miR-34a. In OC cells, overexpression of OIP5-AS1 resulted in the upregulated expression of snail, while overexpression of miR-34a downregulated the expression of snail. In addition, overexpression of miR-34a reduced the effects of overexpression of OIP5-AS1 on the expression of snail. In cell invasion and migration assay, overexpression of OIP5-AS1 and snail resulted in increased OC cell invasion and migration, while overexpression of miR-34a decreased OC cell invasion and migration. Moreover, overexpression of miR-34a attenuated the effects of OIP5-AS1 overexpression on OC cell invasion and migration. Therefore, OIP5-AS1 may upregulate snail expression in OC by sponging miR-34a to promote OC cell invasion and migration.

中文翻译：

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LncRNA OIP5-AS1通过海绵化miR-34a促进卵巢癌细胞的侵袭和迁移来上调蜗牛的表达

尽管OIP5-AS1在许多类型的癌症中均被表征为致癌lncRNA，但其在卵巢癌（OC）中的作用和潜在机制仍然未知。这项研究旨在调查OIP5-AS1在OC中的作用。从58例OC患者（年龄范围36至67岁，平均年龄51.4±5.9岁）中收集了OC组织和非肿瘤组织（肿瘤周围3厘米内的卵巢组织）。通过RT-qPCR确定OIP5-AS1和蜗牛在配对组织中的表达。IntaRNA2.0预测了OIP5-AS1与miR-34a之间的相互作用，并通过双重萤光素酶报告基因检测证实了这一点。通过RT-qPCR和Western印迹分析了OIP5-AS1和miR-34a的过表达对蜗牛表达的影响。通过Transwell测定法分析细胞的侵袭和迁移。我们观察到OC中OIP5-AS1和蜗牛的表达上调并且彼此正相关。RNA-RNA相互作用分析表明，OIP5-AS1可能会抑制miR-34a。在OC细胞中，OIP5-AS1的过度表达导致蜗牛的表达上调，而miR-34a的过度表达则使蜗牛的表达下调。此外，miR-34a的过表达减少了OIP5-AS1的过表达对蜗牛表达的影响。在细胞侵袭和迁移测定中，OIP5-AS1和蜗牛的过度表达导致OC细胞侵袭和迁移增加，而miR-34a的过度表达则降低了OC细胞侵袭和迁移。此外，miR-34a的过表达减弱了OIP5-AS1过表达对OC细胞侵袭和迁移的影响。因此，