Genetic regulation is achieved by monitoring multiple levels of gene expression, from transcription to protein interaction. Unlike common temporary transcription regulation methods such as the use of inducible promoters, integrases permanently edit DNA sequences. Integrases, however, require especially strict regulation when implemented in synthetic genetic systems because of the irreversible result. Here we propose to improve the regulation of site-specific integrase-based genetic system by dynamically hiding one of the attB/P sites that are essential for recombination with transcriptional factors. After effectively suppressing excessive recombination, we also validated the necessity of each of the essential components in our transcription factor-controlled recombination (TFCR) system. The system applied transcription-level regulators directly on controlling the activity of existing non-transcription-level trans-factor proteins by inhibiting its binding to the cis-regulatory elements. We anticipate my results to provide greater robustness for integrase components to enable safer use in systems as well as be a starting point for future cross-expression level gene regulations.

中文翻译：

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转录因子介导的DNA重组动态控制的新型设计。

通过监控从转录到蛋白质相互作用的多个基因表达水平，可以实现遗传调控。与常见的临时转录调控方法（如使用诱导型启动子）不同，它整合了永久编辑的DNA序列。但是，由于无法逆转的结果，在合成遗传系统中进行整合时，需要特别严格的监管。在这里，我们建议通过动态隐藏对转录因子重组必不可少的attB / P位点之一，来改善基于特定位点的整合酶的遗传系统的调控。在有效抑制过度重组后，我们还验证了转录因子控制重组（TFCR）系统中每个基本成分的必要性。该系统通过抑制其与顺式调控元件的结合，直接在控制现有非转录水平反式因子蛋白的活性上应用了转录水平调节剂。我们预计我的结果将为整合酶组件提供更大的鲁棒性，以使在系统中的使用更安全，并成为未来交叉表达水平基因法规的起点。