Mitochondria play key roles in cellular energy metabolism within eukaryotic cells. As relics of endosymbiotic bacteria, most (but not all) mitochondria contain their own genome (mitogenome, mtDNA), as well as intrinsic biosynthetic machinery for making organelle RNAs and proteins. The expression of the mtDNA requires regulated metabolism of its transcriptome by nuclear-encoded factors. Post-transcriptional mtRNA modifications play a central role in the expression of the plant mitogenomes, and hence in organellar biogenesis and plant physiology. Despite extensive investigations, a full map of angiosperm mitochondrial transcriptomes, a prerequisite for the elucidation of the basic RNA biology of mitochondria, has not been reported yet. Using RNA-seq data, RT-PCR and bioinformatics, we sought to explore the gene expression profiles of land plant mitochondria. Here, we present the mitochondrial transcriptomic maps of two key Brassicaceae species, Arabidopsis thaliana (var Col-0) and cauliflower (Brassica oleracea var. botrytis). The revised transcriptome landscapes of Arabidopsis and cauliflower mitogenomes provide with more detail into mtRNA biology and processing in angiosperm mitochondria, and we expect that they would serve as a valuable resource for the plant organellar community.

中文翻译：

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深入了解两个芸苔属植物物种拟南芥（变种Col-0）和甘蓝（变种botrytis）的线粒体转录组景观

线粒体在真核细胞内的细胞能量代谢中起关键作用。作为内共生细菌的遗迹，大多数（但不是全部）线粒体包含其自身的基因组（有丝分裂基因组，mtDNA），以及用于制造细胞器RNA和蛋白质的内在生物合成机制。mtDNA的表达需要通过核编码因子调节其转录组的代谢。转录后的mtRNA修饰在植物有丝分裂基因组的表达中起着核心作用，因此在细胞器生物发生和植物生理学中也起着重要作用。尽管进行了广泛研究，但尚未报道完整的被子植物线粒体转录组图谱，这是阐明线粒体基本RNA生物学的前提。利用RNA序列数据，RT-PCR和生物信息学，我们试图探索陆地植物线粒体的基因表达谱。在这里，我们介绍了两个关键的十字花科物种，拟南芥（var Col-0）和花椰菜（芸苔var。botrytis）的线粒体转录组图。修改后的拟南芥和花椰菜有丝分裂基因组的转录组景观提供了更多有关mtRNA生物学和被子植物线粒体加工的详细信息，我们希望它们将为植物细胞体群落提供宝贵的资源。