Acinetobacter baumannii is the leading cause of hospital-acquired infection in Vietnam. Of note, antibiotic resistance genes are significantly popular in clinical isolates of A. baumannii. Therefore, rapid identification of A. baumannii and determination of antibiotic resistance genes will help to make effective clinical decisions related to antibiotic use. This paper proposes a multiplex PCR to identify Acinetobacter baumannii and detect their beta-lactam antibiotic resistance genes in clinical isolates. Multiplex PCR was applied to amplified recA gene and region ITS 16S - 23S rDNA for Rapid detection of A. baumannii. The two antibiotic resistance genes - blaOXA-51-like, ampC gene - were detected by multiplex PCR and three genes coding Extended-spectrum beta-lactamases - blaCTX-M, blaTEM, blaSHV genes - were subjected to PCR. 49 bacteria strains were subjected to colony PCR. The result showed that 46 strains were A. baumannii and 3 strains belonged to the genus Acinetobacter. The multiplex PCR showed that all of 46 A. baumannii containing the blaOXA-51-like gene and the AmpC gene; 34 strains possess the gene blaTEM and none of them has blaCTX-M and blaSHV genes. The results of the multiplex PCR are the same as those of the in vitro antibiotic sensitivity testing of A. baumannii. However, applying the multiplex PCR directly from the bacteria colony, we can proceed simultaneously with the bacterial identification and the antibiotic resistance gene detection.

中文翻译：

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多重PCR鉴定鲍曼不动杆菌和临床样品中β-内酰胺类抗生素耐药基因。

鲍曼不动杆菌是越南医院获得性感染的主要原因。值得注意的是，抗生素抗性基因在鲍曼不动杆菌的临床分离株中非常流行。因此，快速鉴定鲍曼不动杆菌和确定抗生素抗性基因将有助于做出与抗生素使用相关的有效临床决策。本文提出了一种多重PCR，以鉴定鲍曼不动杆菌并检测其临床分离物中的β-内酰胺类抗生素抗性基因。将多重PCR应用于扩增的recA基因和ITS 16S-23S rDNA区域，以快速检测鲍曼不动杆菌。通过多重PCR检测了两个抗生素抗性基因-blaOXA-51-like，ampC基因，对三个编码扩展谱β-内酰胺酶的基因-blaCTX-M，blaTEM，blaSHV基因进行了PCR。对49个细菌菌株进行菌落PCR。结果表明，鲍曼不动杆菌46株，不动杆菌属3株。多重PCR显示46株鲍曼不动杆菌均含有blaOXA-51样基因和AmpC基因。34个菌株具有blaTEM基因，但都没有blaCTX-M和blaSHV基因。多重PCR的结果与鲍曼不动杆菌的体外抗生素敏感性测试的结果相同。但是，直接从细菌菌落中应用多重PCR，我们可以同时进行细菌鉴定和抗生素抗性基因检测。包含blaOXA-51样基因和AmpC基因的鲍曼氏菌；34个菌株具有blaTEM基因，但都没有blaCTX-M和blaSHV基因。多重PCR的结果与鲍曼不动杆菌的体外抗生素敏感性测试的结果相同。但是，直接从细菌菌落中应用多重PCR，我们可以同时进行细菌鉴定和抗生素抗性基因检测。包含blaOXA-51样基因和AmpC基因的鲍曼氏菌；34个菌株具有blaTEM基因，但都没有blaCTX-M和blaSHV基因。多重PCR的结果与鲍曼不动杆菌的体外抗生素敏感性测试的结果相同。但是，直接从细菌菌落中应用多重PCR，我们可以同时进行细菌鉴定和抗生素抗性基因检测。