The influenza-specific antibody repertoire is continuously reshaped by infection and vaccination. The host immune response to contemporary viruses can be redirected to preferentially boost antibodies specific for viruses encountered early in life, a phenomenon called original antigenic sin (OAS) that is suggested to be responsible for diminished vaccine effectiveness after repeated vaccination. In this study, we used a new computational tool called Neutralization Map to determine the hemagglutination inhibition profiles of individual antibodies within ferret antisera elicited by repeated influenza A/H3 infections. Our results suggest that repeated infections continuously reshape the ferret antibody repertoire, but that a broadly neutralizing antibody signature can nevertheless be induced irrespective of OAS. Overall, our study offers a new way to visualize how immune history shapes individual antibodies within a repertoire, which may help inform future vaccine design.

中文翻译：

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在具有反复流行性感冒A / H3感染的雪貂中绘制抗体库的图谱：原始的抗原罪真的真的“有罪”吗？

流感特异性抗体库通过感染和疫苗接种不断重塑。可以将宿主对当代病毒的免疫反应重定向，以优先增强针对生命早期遇到的病毒的特异性抗体，这种现象被称为原始抗原罪（OAS），这种现象被认为是反复接种疫苗后疫苗效力下降的原因。在这项研究中，我们使用了一种称为中和图的新计算工具来确定由反复感染A / H3流感引起的雪貂抗血清中各个抗体的血凝抑制特性。我们的结果表明，反复感染不断重塑雪貂抗体库，但无论OAS如何，均可诱导广泛中和的抗体特征。总体，