The resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet (UV) radiation dose have been used to differentiate between live and dead bacteria. The enhanced resonance Raman spectra of color-pigmented bacteria were recorded after excitation with visible light diode lasers. In addition, the resonance enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations ( 10^8 cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in-situ was designed, constructed and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses and plants, in-situ, and detect infections within a very short period of time.

中文翻译：

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共振拉曼光谱用于细菌菌株的原位鉴定及其灭活

细菌类胡萝卜素的共振拉曼光谱已被用于鉴定细菌菌株，其强度随紫外线（UV）辐射剂量的变化已被用于区分活细菌和死细菌。用可见光二极管激光器激发后，记录了色素沉着细菌的增强共振拉曼光谱。此外，共振增强的拉曼光谱使我们能够以比通常的光谱学检测低得多的浓度（10 ^ 8细胞/ mL）检测水中的细菌。设计，构造了一种能够现场记录共振拉曼光谱的手持式光谱仪，并用于记录光谱。除细菌外，本文介绍的方法还可用于原位鉴定真菌，病毒和植物。