This work describes a general method for the encapsulation of enzymes with albumin as wall material and the enzyme catalase as prime example. Care was taken for the preparation of biochemically active sub micrometer particles in order to prevent oxygen toxicity induced by artificial oxygen carriers of any type. In cell culture experiments, capsules containing catalase did not exhibit any harmful activities in the absence of peroxides. In the presence of hydrogen peroxide application of low and medium dosed capsules below 0.05 vol% (final concentration 0.001 vol%) even increased the cell damaging process. However, a higher dosage of capsules (> 0.05 vol%) prevented completely cellular disruption induced by 5 mM hydrogen peroxide and decreased up to 90% of cellular damage at higher peroxide concentrations. These results demonstrated that encapsulated catalase was enzymatically activ and the over-all activity of prepared catalase capsules was determined to be > 1000 U ∙ mL_-1 ∙ vol%_-1.

中文翻译：

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酶活性测微蛋白胶囊的合成与表征

这项工作描述了一种以白蛋白为壁材料，以酶过氧化氢酶为主要实例来包封酶的一般方法。为了防止由任何类型的人造氧气载体引起的氧气中毒，要小心地准备具有生化活性的亚微米级颗粒。在细胞培养实验中，含有过氧化氢酶的胶囊在没有过氧化物的情况下没有表现出任何有害活性。在过氧化氢的存在下，低于0.05％（体积）（终浓度为0.001％（体积））的中低剂量胶囊的使用甚至会增加细胞的破坏过程。但是，较高剂量的胶囊（> 0.05 vol％）可防止5 mM过氧化氢引起的细胞完全破坏，并在较高的过氧化物浓度下减少多达90％的细胞损伤。_-1 ∙vol％_-1。