Introduction Skeletal muscle is a major site for whole-body glucose disposal, and determination of skeletal muscle glucose uptake is an important metabolic measurement, particularly in research focussed on interventions that impact muscle insulin sensitivity. Calculating arterial-venous difference in blood glucose can be used as an indirect measure for assessing glucose uptake. However, the possibility of multiple tissues contributing to the composition of venous blood, and the differential in glucose uptake kinetics between tissue types, suggests that sampling from different vein sites could influence the estimation of glucose uptake. This study aimed to determine the impact of venous cannula position on calculated forearm glucose uptake following an oral glucose challenge in resting and post-exercise states. Materials and Methods In 9 young, lean, males, the impact of sampling blood from two antecubital vein positions; the perforating vein (‘perforating’ visit) and, at the bifurcation of superficial and perforating veins (‘bifurcation’ visit), was assessed. Brachial artery blood flow and arterialised-venous and venous blood glucose concentrations were measured in 3 physiological states; resting-fasted, resting-fed, and fed following intermittent forearm muscle contraction (fed-exercise). Results Following glucose ingestion, forearm glucose uptake area under the curve was greater for the ‘perforating’ than for the ‘bifurcation’ visit in the resting-fed (5.92±1.56 vs. 3.69±1.35 mmol/60 min, P<0.01) and fed-exercise (17.38±7.73 vs. 11.40±7.31 mmol/75 min, P<0.05) states. Discussion Antecubital vein cannula position impacts calculated postprandial forearm glucose uptake. These findings have implications for longitudinal intervention studies where serial determination of forearm glucose uptake is required.

中文翻译：

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肘前静脉插管位置影响健康志愿者口服葡萄糖挑战期间前臂葡萄糖摄取的评估

简介 骨骼肌是全身葡萄糖处理的主要场所，骨骼肌葡萄糖摄取的测定是一项重要的代谢测量，特别是在关注影响肌肉胰岛素敏感性的干预措施的研究中。计算血糖的动静脉差异可用作评估葡萄糖摄取的间接措施。然而，多种组织可能有助于静脉血的组成，以及组织类型之间葡萄糖摄取动力学的差异，表明从不同静脉部位取样可能会影响葡萄糖摄取的估计。本研究旨在确定静脉插管位置对静息和运动后口服葡萄糖激发后计算的前臂葡萄糖摄取的影响。材料与方法 在 9 名年轻人中，瘦，男性，从两个肘前静脉位置采血的影响；评估穿支静脉（“穿孔”访问）和浅静脉和穿支静脉分叉处（“分叉”访问）。在 3 种生理状态下测量肱动脉血流量和动脉化静脉和静脉血糖浓度；静息禁食、静息进食和间歇性前臂肌肉收缩后进食（进食运动）。结果 葡萄糖摄入后，“穿孔”的前臂葡萄糖摄取曲线下面积大于“分叉”访视的静息喂养（5.92±1.56 vs. 3.69±1.35 mmol/60 min，P<0.01）和进食运动（17.38±7.73 vs. 11.40±7.31 mmol/75 min，P<0.05）状态。讨论 肘前静脉插管位置影响计算的餐后前臂葡萄糖摄取。