Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1: - 9: were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5: ) and quercetin 3-O-(6-O-malonyl)-β-D-glucopyranoside (6: ) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6: . Niazirin (2: ) was detected in amounts between 0.010 - 0.049 mg/100 mg while compound 1: was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

中文翻译：

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通过 UHPLC-PDA-MS 分析和量化鼓槌树（辣木）和膳食补充剂中的关键植物化学物质

辣木被称为鼓槌树，栽培于亚热带和热带地区。它具有抗高血压和抗糖尿病作用。建立了一种超高效液相色谱法，用于测定 M. oleifera 叶子和上市产品中的 9 种植物化学物质。使用 C-18 色谱柱作为固定相，水/乙腈和 0.05% 甲酸作为流动相，在 45 °C 的温度下在 7 分钟内实现了高效分离。对该方法的线性、重复性、检测限和定量限进行了验证。酚类化合物 1: - 9: 的检测限低至 0.2 µg/mL。招募了 220 和 255 nm 波长的光电二极管阵列检测器进行量化。关键植物化学物质的检测范围为 0.42 至 2。13 种膳食补充剂中的 57 毫克/100 毫克样品重量。本研究首次考虑了对油菜中木脂素的定量分析。异槲皮苷 (5: ) 和槲皮素 3-O-(6-O-丙二酰基)-β-D-吡喃葡萄糖苷 (6: ) 在 M. oleifera 的叶子中占主导地位，化合物 6: 具有固有的可降解性质。Niazirin (2: ) 的检测量在 0.010 - 0.049 mg/100 mg 之间，而化合物 1: 由于分馏过程而无法检测到并且可能是伪影。采用液相色谱-电喷雾电离-质谱法对正离子和负离子模式进行萃取离子监测，对组分进行表征和确认。