Disjunction between canola distribution and the genetic structure of its recently described pest, the canola flower midge (Contarinia brassicola),Ecology and Evolution

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Disjunction between canola distribution and the genetic structure of its recently described pest, the canola flower midge (Contarinia brassicola)
Ecology and Evolution ( IF 2.3 ) Pub Date : 2020-10-26 , DOI: 10.1002/ece3.6927
Erin O Campbell ₁ , Julian R Dupuis ₂ , Jennifer Holowachuk ₃ , Shane Hladun ₃ , Meghan A Vankosky ₃ , Boyd A Mori _{1,

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Affiliation

Population genomics is a useful tool to support integrated pest management as it can elucidate population dynamics, demography, and histories of invasion. Here, we use a restriction site‐associated DNA sequencing approach combined with whole‐genome amplification (WGA) to assess genomic population structure of a newly described pest of canola, the diminutive canola flower midge, Contarinia brassicola. Clustering analyses recovered little geographic structure across the main canola production region but differentiated several geographically disparate populations at edges of the agricultural zone. Given a lack of alternative hypotheses for this pattern, we suggest these data support alternative hosts for this species and thus our canola‐centric view of this midge as a pest has limited our understanding of its biology. These results speak to the need for increased surveying efforts across multiple habitats and other potential hosts within Brassicaceae to improve both our ecological and evolutionary knowledge of this species and contribute to effective management strategies. We additionally found that use of WGA prior to library preparation was an effective method for increasing DNA quantity of these small insects prior to restriction site‐associated DNA sequencing and had no discernible impact on genotyping consistency for population genetic analysis; WGA is therefore likely to be tractable for other similar studies that seek to randomly sample markers across the genome in small organisms.

中文翻译：

双低油菜籽分布与其最近描述的害虫双低油菜花蠓 (Contarinia bassicola) 的遗传结构之间的脱节

种群基因组学是支持害虫综合管理的有用工具，因为它可以阐明种群动态、人口统计和入侵历史。在这里，我们使用限制性位点相关的 DNA 测序方法结合全基因组扩增 (WGA) 来评估一种新描述的油菜害虫（小型油菜花蠓）的基因组种群结构。聚类分析几乎没有恢复整个油菜籽主产区的地理结构，但区分了农业区边缘的几个地理上不同的种群。鉴于这种模式缺乏替代假设，我们建议这些数据支持该物种的替代宿主，因此我们以双低油菜为中心的将这种蠓视为害虫的观点限制了我们对其生物学的理解。这些结果表明需要加大对十字花科内多个栖息地和其他潜在寄主的调查力度，以提高我们对该物种的生态和进化知识，并为有效的管理策略做出贡献。我们还发现，在文库制备之前使用 WGA 是在限制性位点相关 DNA 测序之前增加这些小昆虫 DNA 数量的有效方法，并且对群体遗传分析的基因分型一致性没有明显影响；因此，WGA 可能适合其他类似的研究，这些研究寻求在小型生物体的基因组中随机采样标记。

更新日期：2020-12-03

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