In this study, a new magnetic nanogel was synthesized for the extraction/pre-concentration of doxorubicin (DOX) from biological samples. The synthesized nano-sorbent was used for the pre-concentration and extraction of DOX prior to spectrophotometry determination. The influence of the main variables, including pH, sorption time, adsorbent amount, and NaCl content, was investigated and optimized by central composite design (CCD) as a response surface methodology. The optimum values obtained for each investigated variable to reach the maximum recovery are pH 5.0, sorption time = 22 min, sorbent amount = 15 mg, and NaCl content = 0%. Under these conditions, the proposed procedure represented good linearity over the range of 0.5–15 µg mL⁻¹ (R² = 0.9991) for water samples, 2–30 µg mL⁻¹ (R² = 0.9977) for urine samples, and 0.6–15 µg mL⁻¹ (R² = 0.9987) for plasma samples. A comparison of the analytical merits of our method with some other published spectroscopic methods for the determination of DOX shows that the developed method is good and comparable with that of other reported methods. The accuracy of the method is confirmed by comparing the results with those obtained by analyzing the samples using a standard method.

在这项研究中，合成了一种新的磁性纳米凝胶，用于从生物样品中提取/预富集阿霉素（DOX）。合成的纳米吸附剂在分光光度法测定之前用于DOX的预浓缩和萃取。通过中央复合设计（CCD）作为响应面方法，研究并优化了主要变量（包括pH，吸附时间，吸附剂含量和NaCl含量）的影响。为获得最大回收率而为每个研究变量获得的最佳值为pH 5.0，吸附时间= 22分钟，吸附剂量= 15 mg和NaCl含量= 0％。在这些条件下，拟议的程序在0.5–15 µg mL ^-1（R ² 水样品为= 0.9991）， 尿液样品为2–30 µg mL ^-1（R ² = 0.9977  ），血浆样品为0.6–15 µg mL ^-1（R ² = 0.9987）。将我们的方法与其他已发表的光谱法测定DOX的分析优点进行比较，结果表明，该方法很好，可与其他报道的方法进行比较。通过将结果与使用标准方法分析样品获得的结果进行比较，可以确认该方法的准确性。