Flowering time has a critically important effect on the reproduction of plants, and many components involved in flowering-time regulation have been identified in multiple plant species. However, studies of the flowering-time genes in mungbean (Vigna radiata) have been limited. Here, we characterized a novel mungbean gene, VrLELP, involved in flowering-time regulation in transgenic Arabidopsis. Subcellular localization analysis revealed that VrLELP was localized in the membrane, cytoplasm and nucleus and the nucleus and membrane contained higher signal than cytoplasm, similar to the empty vector control. The expression of VrLELP was higher in leaves and pods and lower in nodule roots relative to other tissues. The expression of VrLELP varied during flower development. The expression of VrLELP also varied during the day, reaching a peak after 12 h of illumination under long-day conditions. In contrast, under short-day conditions, the abundance of VrLELP transcripts changed little throughout the day. In addition, VrLELP delayed flowering time in transgenic Arabidopsis plants by suppressing the expression of the flowering-time genes CO and FT under short-day conditions. However, VrLELP did not affect flowering time under long-day conditions in Arabidopsis. Our study provides essential information for future studies of the molecular mechanisms of the flowering-time regulation system in mungbean.

开花时间对植物的繁殖具有至关重要的影响，并且已经在多种植物物种中鉴定出许多参与开花时间调节的成分。然而，对绿豆（ Vigna radiata ）开花时间基因的研究一直很有限。在这里，我们描述了一种新的绿豆基因VrLELP，它参与转基因拟南芥的开花时间调控。亚细胞定位分析表明，VrLELP 定位于细胞膜、细胞质和细胞核，细胞核和细胞膜信号高于细胞质，与空载体对照相似。相对于其他组织， VrLELP的表达在叶和豆荚中较高，而在根瘤根中较低。VrLELP的表达在花发育过程中发生变化。VrLELP的表达在白天也有所不同，在长日照条件下光照 12 小时后达到峰值。相比之下，在短日照条件下，VrLELP转录本的丰度全天变化不大。此外，VrLELP在短日照条件下通过抑制开花时间基因CO和FT的表达来延迟转基因拟南芥植物的开花时间。然而，VrLELP并不影响拟南芥在长日照条件下的开花时间。我们的研究为今后研究绿豆开花时间调控系统的分子机制提供了必要的信息。