Ibervillea sonorae (S. Watson) Greene, (huereque) is an endemic shrub whose roots are used in traditional medicine to treat diabetes. Here, we establish cell cultures of I. sonorae to evaluate the presence of antidiabetic metabolites. Stem and roots sections were incubated on media with different concentrations of growth regulators to induce calli. Using B5 medium containing 25 g L−1 sucrose, 150 mg L−1ascorbic acid, 6 g L−1 agar–agar, supplemented with 0.5 mg L−1 α-naphthalene-acetic acid, 0.5 mg L−1 benzyladenine and 1 mg L−1 indole-3-acetic acid, 95% calli induction was attained. Flavonoids and phenols in I. sonorae cultured cell suspension were investigated. The methanol extracts exhibited antioxidant activity that was attributed to phenolic compounds. These extracts stimulated glucose uptake more efficaciously than I. sonorae plant roots. This study demonstrates that in vitro cultured cells stand as a good option for production of hypoglycemic metabolites.

中文翻译：

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与植物根部相比，Ibervillea sonoae (S. Watson) Greene 培养细胞中的代谢物显示出更高的降血糖活性

Ibervillea sonorae (S. Watson) Greene, (huereque) 是一种地方性灌木，其根在传统医学中用于治疗糖尿病。在这里，我们建立了 I.sonorae 的细胞培养物，以评估抗糖尿病代谢物的存在。茎和根切片在含有不同浓度生长调节剂的培养基上培养以诱导愈伤组织。使用含有 25 g L-1 蔗糖、150 mg L-1 抗坏血酸、6 g L-1 琼脂的 B5 培养基，并辅以 0.5 mg L-1 α-萘乙酸、0.5 mg L-1 苄基腺嘌呤和 1 mg L-1 吲哚-3-乙酸，达到 95% 愈伤组织诱导。研究了 I.sonorae 培养的细胞悬浮液中的类黄酮和酚类。甲醇提取物表现出归因于酚类化合物的抗氧化活性。这些提取物比 I.sonorae 植物根更有效地刺激葡萄糖吸收。