CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) is one of the hallmark of biological tools, contemplated as a valid and hopeful alternatives to genome editing. Advancements in CRISPR-based technologies have empowered scientists with an editing kit that allows them to employ their knowledge for deleting, replacing and lately “Gene Surgery”, and provides unique control over genes in broad range of species, and presumably in humans. These fast-growing technologies have high strength and flexibility and are becoming an adaptable tool with implementations that are altering organism’s genome and easily used for chromatin manipulation. In addition to the popularity of CRISPR in genome engineering and modern biology, this major tool authorizes breakthrough discoveries and methodological advancements in science. As scientists are developing new types of experiments, some of the applications are raising questions about what CRISPR can enable. The results of evidence-based research strongly suggest that CRISPR is becoming a practical tool for genome-engineering and to create genetically modified eukaryotes, which is needed to establish guidelines on new regulatory concerns for scientific communities.

CRISPR（成簇规则间隔短回文重复序列）是生物工具的标志之一，被认为是基因组编辑的有效且充满希望的替代方案。基于 CRISPR 技术的进步为科学家提供了一种编辑套件，使他们能够利用自己的知识进行删除、替换和最近的“基因手术”，并为广泛物种（可能包括人类）的基因提供独特的控制。这些快速发展的技术具有高强度和灵活性，正在成为一种适应性强的工具，其实施可以改变生物体的基因组并易于用于染色质操作。除了 CRISPR 在基因组工程和现代生物学中的普及之外，这一主要工具还促进了科学领域的突破性发现和方法论进步。随着科学家们开发新型实验，一些应用引发了人们对 CRISPR 功能的质疑。循证研究的结果强烈表明，CRISPR 正在成为基因组工程和创建转基因真核生物的实用工具，这是为科学界制定新监管问题的指导方针所必需的。