Osteosarcoma(OS) is the most common bone malignancy in both children and adolescents. In this study, we aimed to explore the association of miRNA-122 and miRNA-96 expression with the clinical characteristics and prognosis of patients with osteosarcoma. The expression of miRNA-122 and miRNA-96 in human osteosarcoma cell lines and tissues were detected in this study. Reverse transcriptase-PCR(RT-PCR) was used to determine the expression levels of miRNA-122 and miRNA-96 in 68 human OS samples. We found that MiRNA-122 and miRNA-96 were widely up-regulated in osteosarcoma, gastric cancer and pancreatic cancer. In HOS, Saos-2 and U2OS osteosarcoma cells, miRNA-122 and miRNA-96 were up-regulated significantly, while down-regulated in MG-63 cells. After further investigation, we found that miRNA-122 and miRNA-96 concentrations were significantly higher in the tumor tissues than those in the normal tissues(ᵄ3;< 0.01). Moreover, the cell proliferation of LV- miRNA-122-RNAi and LV- miRNA-96-RNAi transfected SaOS2 were significantly decreased compared to the LV- miRNA-122-RNAi-CN and LV- miRNA-96-RNAi group. After adjusting for competing risk factors, we found combined high miRNA-122 and miRNA-96 expression was identified as independent predictor of overall survival.

中文翻译：

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微小RNA 122和微小RNA 96影响人类骨肉瘤的生物学行为，并与骨肉瘤患者的预后相关。

骨肉瘤（OS）是儿童和青少年中最常见的骨恶性肿瘤。在这项研究中，我们旨在探讨miRNA-122和miRNA-96表达与骨肉瘤患者的临床特征和预后的关系。本研究检测了miRNA-122和miRNA-96在人骨肉瘤细胞系和组织中的表达。逆转录-聚合酶链反应（RT-PCR）用于确定68个人类OS样品中miRNA-122和miRNA-96的表达水平。我们发现，miRNA-122和miRNA-96在骨肉瘤，胃癌和胰腺癌中被广泛上调。在HOS，Saos-2和U2OS骨肉瘤细胞中，miRNA-122和miRNA-96显着上调，而在MG-63细胞中则下调。经过进一步调查 我们发现肿瘤组织中的miRNA-122和miRNA-96的浓度显着高于正常组织（ᵄ3； <0.01）。此外，与LV-miRNA-122-RNAi-CN和LV-miRNA-96-RNAi组相比，LV-miRNA-122-RNAi和LV-miRNA-96-RNAi转染的SaOS2的细胞增殖显着降低。在调整了竞争性危险因素后，我们发现结合的高miRNA-122和miRNA-96表达被确定为总体生存的独立预测因子。