[Background] Sphingosine-1-phosphate (S1P) plays a significant role in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). [Methods] We collected the plasma samples from 40 patients with AAV and 10 healthy volunteers. The plasma levels of S1P were tested by enzyme-linked immunosorbent assay (ELISA). The levels of serum creatinine (Scr) were tested by rate method, and then the glomerular filtration rate (eGFR) of the patients was calculated from the Scr, age, and gender. Prothrombin time (PT), partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB), fibrinogen reduction product (FDP), D-dimer and C-reactive protein (CRP) were tested by turbidimetric inhibition immunoassays. Platelets (PLT) were tested by fluorescently-labelled electrical impedance method. [Results] The plasma levels of S1P was significantly higher in AAV patients than in healthy volunteers. Correlation analysis showed that plasma levels of S1P were negatively correlated with glomerular filtration (p=0.022, r=-0.306), and positively correlated with circulating levels of Birmingham vasculitis activity score (BVAS), PLT and D-dimer, (p=0.004, r=0.443; p<0.001, r=0.654; p=0.006, r=0.427). The 40 patients with AAV were classified into three groups: the thromboembolism group (with complications of cerebral infarction and myocardial infarction, n=6), cerebral ischemia group (n=4), and cerebral hemorrhage group (n=2). The plasma levels of S1P were highest in the thromboembolism group and lowest in the cerebral hemorrhage group (p=0.003). [Conclusions] Plasma levels of S1P were associated with circulating levels of D-dimer, PLT, and BVAS in the patients with AAV. Hence, plasma S1P level can be used as a biomarker to predict coagulation-related complications in AAV.

中文翻译：

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抗中性粒细胞胞浆抗体相关血管炎中的鞘氨醇-1-磷酸：凝血相关的临床指标和并发症。

[背景] 1-磷酸鞘氨醇（S1P）在抗中性粒细胞胞浆抗体（ANCA）相关血管炎（AAV）中起着重要作用。方法收集40例AAV患者和10例健康志愿者的血浆样本。S1P的血浆水平通过酶联免疫吸附试验（ELISA）进行测试。用比率法检测血清肌酐（Scr）水平，然后根据Scr，年龄和性别计算出患者的肾小球滤过率（eGFR）。通过比浊抑制免疫分析法检测凝血酶原时间（PT），部分凝血活酶时间（APTT），凝血酶时间（TT），纤维蛋白原（FIB），纤维蛋白原还原产物（FDP），D-二聚体和C反应蛋白（CRP）。血小板（PLT）通过荧光标记的电阻抗方法进行测试。[结果] AAV患者的血浆S1P水平显着高于健康志愿者。相关分析表明，血浆S1P水平与肾小球滤过率呈负相关（p = 0.022，r = -0.306），与伯明翰血管炎活性评分（BVAS），PLT和D-二聚体的循环水平呈正相关（p = 0.004）。 ，r ＝ 0.443； p ＜0.001，r ＝ 0.654； p ＝ 0.006，r ＝ 0.427）。40例AAV患者分为三组：血栓栓塞组（合并有脑梗死和心肌梗塞，n = 6），脑缺血组（n = 4）和脑出血组（n = 2）。血栓栓塞组的血浆S1P水平最高，脑出血组的血浆S1P水平最低（p = 0.003）。[结论]血浆S1P水平与D-二聚体，PLT，AAV患者的BVAS和BVAS。因此，血浆S1P水平可以用作预测AAV中凝血相关并发症的生物标志物。