Evaluation of the probiotic and functional potential of Lactobacillus agilis 32 isolated from pig manure,Letters in Applied Microbiology

当前位置： X-MOL 学术 › Lett. Appl. Microbiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Evaluation of the probiotic and functional potential of Lactobacillus agilis 32 isolated from pig manure
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-10-24 , DOI: 10.1111/lam.13422
S Shi _{1,

2} , B Cheng ₁ , B Gu ₁ , T Sheng ₁ , J Tu ₁ , Y Shao ₁ , K Qi ₁ , D Zhou ₂

Affiliation

Escherichia coli is a symbiotic bacterium in humans and animals and an important pathogen of humans and animals. Prevention and suppression of E. coli infection is of great concern. In this study, we isolated a strain of L. agilis 32 from pig manure and evaluated its biological characteristics, and found that its bacterial survival rate was 25% after 4 hours of treatment at pH 2, and under the condition of 0.5% bile concentration, its survival rate exceeds 30%. In addition, L. agilis 32 has a cell surface hydrophobicity of 77.8%, and exhibits 67.1% auto-aggregation and 63.2% aggregation with Enterotoxigenic Escherichia coli 10 (ETEC 10). FITC fluorescence labeling showed that the fluorescence intensity of cecum was significantly higher than that of duodenum, jejunum or colon (P < 0.05), but no significant difference from ileum. L. agilis 32 bacterial culture and CFS showed average inhibition zone diameters of 14.2 mm and 15.4 mm, respectively. L. agilis 32 CFS treatment can significantly reduce the pathogenicity of ETEC 10. These results show that L. agilis 32 is an active and potential probiotic, and it has a good antibacterial effect on ETEC10, which provides basic research for probiotics to prevent and treat intestinal diarrhea pathogen infection.

中文翻译：

评估从猪粪中分离的敏捷乳杆菌 32 的益生菌和功能潜力

大肠杆菌是人和动物的共生菌，是人和动物的重要病原体。预防和抑制大肠杆菌感染备受关注。本研究从猪粪中分离出一株 L. agilis 32 并对其生物学特性进行了评价，发现在 pH 2、胆汁浓度 0.5% 条件下处理 4 小时后，其细菌存活率为 25% ，其存活率超过30%。此外，L. agilis 32 的细胞表面疏水性为 77.8%，并且与产肠毒素大肠杆菌 10 (ETEC 10) 的自动聚集和聚集分别为 67.1% 和 63.2%。FITC荧光标记显示盲肠的荧光强度显着高于十二指肠、空肠或结肠（P < 0.05），但与回肠无显着差异。L。agilis 32 细菌培养物和 CFS 的平均抑菌圈直径分别为 14.2 毫米和 15.4 毫米。L. agilis 32 CFS处理可以显着降低ETEC 10的致病性。这些结果表明L. agilis 32是一种活性和潜在的益生菌，对ETEC10具有良好的抗菌作用，为益生菌防治提供基础研究肠道腹泻病原体感染。

更新日期：2020-10-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11