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GDF15 knockdown suppresses cervical cancer cell migration in vitro through the TGF‐β/Smad2/3/Snail1 pathway
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-10-23 , DOI: 10.1002/2211-5463.13013 Li Li 1 , Ruihong Zhang 2 , Hailei Yang 3 , Donghua Zhang 4 , Jiwei Liu 4 , Jinfang Li 5 , Bin Guo 5
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-10-23 , DOI: 10.1002/2211-5463.13013 Li Li 1 , Ruihong Zhang 2 , Hailei Yang 3 , Donghua Zhang 4 , Jiwei Liu 4 , Jinfang Li 5 , Bin Guo 5
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Growth differentiation factor 15 (GDF15), a member of the transforming growth factor β (TGF‐β) superfamily, is a prognostic biomarker of cervical cancer. In addition, GDF15 has been reported to enhance the migration of colorectal cancer cells and liver cancer stem‐like cells. However, the mechanism by which GDF15 promotes cervical cancer cell migration is not completely understood. Here, we report that GDF15 expression is enhanced in cervical cancer tissues, as well as in cultured cervical cancer cells. ShGDF15 transfection markedly inhibited expression of Vimentin, N‐cadherin and Snail1, and resulted in up‐regulation of E‐cadherin expression in HT‐3 and HeLa cells. Moreover, knockdown of GDF15 suppressed wound healing rate and reduced the number of invasive cells. Furthermore, knockdown of GDF15 significantly suppressed the expression of phosphorylated Smad2 and Smad3. The addition of TGF‐β1 partially abolished the inhibitory effects of GDF15 knockdown on the migration and invasion of cervical cancer cells. In summary, we report here that GDF15 knockdown inhibits migration and invasion of cervical cancer cells in vitro through the TGF‐β/Smad2/3/Snail1 pathway.
中文翻译：
GDF15敲低通过TGF-β/Smad2/3/Snail1通路抑制体外宫颈癌细胞迁移
生长分化因子 15 (GDF15) 是转化生长因子 β (TGF-β) 超家族的成员，是宫颈癌的预后生物标志物。此外，据报道，GDF15 可增强结直肠癌细胞和肝癌干细胞样细胞的迁移。然而，GDF15 促进宫颈癌细胞迁移的机制尚不完全清楚。在这里，我们报告 GDF15 表达在宫颈癌组织以及培养的宫颈癌细胞中增强。ShGDF15 转染显着抑制波形蛋白、N-钙粘蛋白和 Snail1 的表达，并导致 HT-3 和 HeLa 细胞中 E-钙粘蛋白的表达上调。此外，GDF15 的敲低抑制了伤口愈合率并减少了侵袭性细胞的数量。此外，GDF15 的敲低显着抑制了磷酸化 Smad2 和 Smad3 的表达。TGF-β1的加入部分消除了GDF15敲低对宫颈癌细胞迁移和侵袭的抑制作用。总之，我们在此报告 GDF15 敲低抑制宫颈癌细胞的迁移和侵袭在体外通过 TGF-β/Smad2/3/Snail1 通路。
更新日期：2020-12-03
中文翻译：
GDF15敲低通过TGF-β/Smad2/3/Snail1通路抑制体外宫颈癌细胞迁移
生长分化因子 15 (GDF15) 是转化生长因子 β (TGF-β) 超家族的成员，是宫颈癌的预后生物标志物。此外，据报道，GDF15 可增强结直肠癌细胞和肝癌干细胞样细胞的迁移。然而，GDF15 促进宫颈癌细胞迁移的机制尚不完全清楚。在这里，我们报告 GDF15 表达在宫颈癌组织以及培养的宫颈癌细胞中增强。ShGDF15 转染显着抑制波形蛋白、N-钙粘蛋白和 Snail1 的表达，并导致 HT-3 和 HeLa 细胞中 E-钙粘蛋白的表达上调。此外，GDF15 的敲低抑制了伤口愈合率并减少了侵袭性细胞的数量。此外，GDF15 的敲低显着抑制了磷酸化 Smad2 和 Smad3 的表达。TGF-β1的加入部分消除了GDF15敲低对宫颈癌细胞迁移和侵袭的抑制作用。总之，我们在此报告 GDF15 敲低抑制宫颈癌细胞的迁移和侵袭在体外通过 TGF-β/Smad2/3/Snail1 通路。
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