Silk fibroin (SF), which is a protein‐based biomacromolecule, proved to be an important biomaterial in biomedical applications and tissue engineering. The present study mainly focused to investigate the effective antibiotic against the common pathogens found at the site of burn wounds. The SF films prepared with selected antibiotics had shown activity against the target bacterial pathogens, but no activity was found against fungal species. The release of antibiotics from films resulted in the microbial susceptibility. Results have shown Ciprofloxacin—6 μg, Amoxicillin—15 μg, and Nystatin—70 μg at minimal inhibition concentration (MIC) has shown maximum zone of inhibition when compared with other antibiotics. The antibiotic‐based silk fibroin (ABSF) films have shown a positive effect on cell viability and cell adhesion on the L929 fibroblast cell line. The structural conformations of films were also carried out by FTIR and XRD, an increase in the crystalline size was observed in the antibiotic‐based film. Results demonstrate that ABSF films can act against the pathogens without losing their drug nature, can reduce the chance of infections at the site of the wound, and also can establish the basis for the accelerated healing process of the burn wound.

中文翻译：

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基于抗生素的丝素蛋白膜可用于烧伤创面愈合

丝素蛋白（SF）是一种基于蛋白质的生物大分子，被证明是生物医学应用和组织工程中的重要生物材料。本研究主要集中于研究针对烧伤创面部位常见病原体的有效抗生素。用选定的抗生素制备的SF膜对靶细菌病原体显示出活性，但对真菌种类没有活性。薄膜中抗生素的释放导致了微生物的敏感性。结果显示，与其他抗生素相比，最小抑菌浓度（MIC）的环丙沙星-6μg，阿莫西林-15μg和制霉菌素-70μg表现出最大的抑菌圈。基于抗生素的丝素蛋白（ABSF）膜已对L929成纤维细胞系的细胞活力和细胞粘附产生了积极影响。薄膜的结构构象也通过FTIR和XRD进行，在基于抗生素的薄膜中观察到晶体尺寸的增加。结果表明，ABSF膜可以对抗病原体而不会失去其药物性质，可以减少伤口部位的感染机会，还可以为烧伤伤口的加速愈合过程奠定基础。