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Direct binding of benzoate derivatives to two chemoreceptors with Cache sensor domains in Halomonas titanicae KHS3
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-10-24 , DOI: 10.1111/mmi.14630
Ana F Gasperotti 1, 2 , M Karina Herrera Seitz 1 , Rocío S Balmaceda 3 , Luciano M Prosa 1 , Kirsten Jung 2 , Claudia A Studdert 3
Affiliation  

Halomonas titanicae KHS3, isolated from a hydrocarbon‐contaminated sea harbor in Argentina, is able to grow on aromatic hydrocarbons and displays chemotaxis toward those compounds. This behavior might contribute to the efficiency of its degradation capacity. Using high throughput screening, we identified two chemoreceptors (Htc1 and Htc2) that bind benzoate derivatives and other organic acids. Whereas Htc1 has a high affinity for benzoate (Kd 112 µM) and 2‐hydroxybenzoate (Kd 83 µM), Htc2 binds 2‐hydroxybenzoate with low affinity (Kd 3.25 mM), and also C3/C4 dicarboxylates. Both chemoreceptors are able to trigger a chemotactic response of E. coli cells to the specific ligands. A H. titanicae htc1 mutant has reduced chemotaxis toward benzoate, and is complemented upon expression of the corresponding receptor. Both chemoreceptors have a Cache‐type sensor domain, double (Htc1) or single (Htc2), and their ability to bind aromatic compounds is reported here for the first time.

中文翻译:

苯甲酸盐衍生物与钛酸单胞菌 KHS3 中具有 Cache 传感器结构域的两个化学感受器的直接结合

Halomonas titanicae KHS3 从阿根廷被碳氢化合物污染的海港中分离出来,能够在芳香烃上生长,并对这些化合物显示出趋化性。这种行为可能有助于提高其降解能力的效率。使用高通量筛选,我们确定了结合苯甲酸酯衍生物和其他有机酸的两种化学感受器(Htc1 和 Htc2)。而Htc1具有用于苯甲酸(K具有高亲和性d 112μM)和2-羟基苯甲酸(K d 83μM),Htc2结合-2-羟基苯以低亲和力(K d 3.25毫摩尔),并且还C3 / C4二羧酸酯。两种化学感受器都能够触发大肠杆菌细胞对特定配体的趋化反应。A H. titanicae htc1突变体对苯甲酸盐的趋化性降低,并在相应受体表达后得到补充。两种化学感受器都具有 Cache 型传感器域,双(Htc1)或单(Htc2),它们结合芳香族化合物的能力在此首次报道。
更新日期:2020-10-24
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