Biodegradable poly(ε‐caprolactone) (PCL) scaffolds with adipose‐derived mesenchymal stem cells (ADSCs) have been used in vascular regeneration studies. An evaluation method of the effect of PCL degradation products (DP) on the viability, stemness, and differentiation capacities of ADSCs is established. ADSCs are cultured in medium containing different concentrations of PCL DP before evaluating the effect of PCL DP on the cell apoptosis and proliferation, cell surface antigens, adipogenic and osteogenic differentiation capacities, and capacities to differentiate into endothelial cells and smooth muscle cells. The results demonstrate that PCL DP exceed 0.05 mg mL⁻¹ may change the stemness and differentiation capacities of ADSCs. Therefore, to control the proper concentration of PCL DP is essential for ADSCs in vascular regeneration application.

中文翻译：

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控制聚（ε-己内酯）降解以维持脂肪源性间充质干细胞在血管再生应用中的干性和功能

带有脂肪间充质干细胞 (ADSC) 的可生物降解聚（ε-己内酯）（PCL）支架已用于血管再生研究。建立了PCL降解产物(DP)对ADSCs活力、干性和分化能力影响的评价方法。在评估 PCL DP 对细胞凋亡和增殖、细胞表面抗原、成脂和成骨分化能力以及分化为内皮细胞和平滑肌细胞的能力的影响之前，将 ADSC 在含有不同浓度 PCL DP 的培养基中培养。结果表明 PCL DP 超过 0.05 mg mL ^-1可能会改变 ADSCs 的干性和分化能力。因此，控制适当的 PCL DP 浓度对于 ADSCs 在血管再生应用中是必不可少的。