Large colloidal probes for atomic force microscopy: Fabrication and calibration issues,Journal of Molecular Recognition

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Large colloidal probes for atomic force microscopy: Fabrication and calibration issues
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2020-10-24 , DOI: 10.1002/jmr.2879
Matteo Chighizola ₁ , Luca Puricelli ₁ , Ludovic Bellon ₂ , Alessandro Podestà ₁

Affiliation

Atomic force microscopy (AFM) is a powerful tool to investigate interaction forces at the micro and nanoscale. Cantilever stiffness, dimensions and geometry of the tip can be chosen according to the requirements of the specific application, in terms of spatial resolution and force sensitivity. Colloidal probes (CPs), obtained by attaching a spherical particle to a tipless (TL) cantilever, offer several advantages for accurate force measurements: tunable and well‐characterisable radius; higher averaging capabilities (at the expense of spatial resolution) and sensitivity to weak interactions; a well‐defined interaction geometry (sphere on flat), which allows accurate and reliable data fitting by means of analytical models. The dynamics of standard AFM probes has been widely investigated, and protocols have been developed for the calibration of the cantilever spring constant. Nevertheless, the dynamics of CPs, and in particular of large CPs, with radius well above 10 μm and mass comparable, or larger, than the cantilever mass, is at present still poorly characterized. Here we describe the fabrication and calibration of (large) CPs. We describe and discuss the peculiar dynamical behaviour of CPs, and present an alternative protocol for the accurate calibration of the spring constant.

中文翻译：

用于原子力显微镜的大型胶体探针：制造和校准问题

原子力显微镜 (AFM) 是研究微观和纳米尺度相互作用力的有力工具。悬臂刚度、尖端的尺寸和几何形状可以根据特定应用的要求在空间分辨率和力灵敏度方面进行选择。胶体探针 (CP) 是通过将球形颗粒附着到无尖端 (TL) 悬臂上而获得的，它为精确的力测量提供了几个优点：可调节且可良好表征的半径；更高的平均能力（以空间分辨率为代价）和对弱相互作用的敏感性；定义明确的交互几何（平面上的球体），允许通过分析模型进行准确可靠的数据拟合。标准 AFM 探针的动力学已被广泛研究，并且已经开发了用于校准悬臂弹簧常数的协议。然而，CPs 的动力学，特别是半径远高于 10 μm 且质量与悬臂质量相当或更大的大型 CPs，目前仍然很难表征。在这里，我们描述了（大）CP 的制造和校准。我们描述和讨论了 CP 的特殊动力学行为，并提出了一种用于精确校准弹簧常数的替代方案。

更新日期：2020-12-04

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