Hyperbranched polyglycerol (HPG) is a biocompatible polyether polymer that is a potential colloid component in a preservation solution for suppressing interstitial edema during cold storage of a donor organ. This study evaluated the outcomes of kidney transplants after cold perfusion and storage with a HPG‐based preservation solution (HPGS) in a pig model of kidney autotransplantation. The left kidneys of farm pigs (weighing 35–45 kg) were perfused with and stored in either cold HPGS or standard UW solution (UWS), followed by transplantation to the right side after right nephrectomy. The survival and function of transplants were determined by the urine output, and serum creatinine (SCr) and blood urea nitrogen (BUN) of recipients. Transplant injury was examined by histological analysis. Here, we showed that there was no significant difference between HPGS and UWS in the prevention of tissue edema, but HPGS was more effective than UWS for initial blood washout of kidney perfusion and for the prevention of cold ischemia injury during cold storage. After autotransplantation, the kidneys preserved with HPGS (HPG group) had better functional recovery than those with UWS (UW group), indicated by significantly more urine output and lower levels of SCr and BUN. The survived grafts in HPG group had less tissue damage than those in UW group. In conclusion, as compared to the UWS the HPGS has less negative impact on kidney cold ischemia during cold storage, resulting in improving immediate functional recovery after transplantation, suggesting that HPG is a promising colloid for donor kidney preservation.

中文翻译：

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超支化聚甘油在自体肾移植猪模型中用于供肾冷灌注和储存的评价

超支化聚甘油 (HPG) 是一种生物相容性聚醚聚合物，是保存液中潜在的胶体成分，用于在供体器官冷藏期间抑制间质水肿。本研究评估了在自体肾移植猪模型中使用基于 HPG 的保存液 (HPGS) 进行冷灌注和储存后的肾移植结果。将农场猪（重 35-45 公斤）的左肾灌注并储存在冷 HPGS 或标准 UW 溶液 (UWS) 中，然后在右肾切除术后移植到右侧。移植物的存活和功能取决于受者的尿量、血清肌酐（SCr）和血尿素氮（BUN）。通过组织学分析检查移植损伤。这里，我们发现HPGS和UWS在预防组织水肿方面没有显着差异，但HPGS在肾脏灌注的初始血液冲洗和冷藏期间预防冷缺血损伤方面比UWS更有效。自体移植后，HPGS 保存的肾脏（HPG 组）比 UWS 保存的肾脏（UW 组）具有更好的功能恢复，表现为尿量显着增加和 SCr 和 BUN 水平降低。HPG组成活移植物的组织损伤较UW组少。总之，与 UWS 相比，HPGS 在冷藏期间对肾脏冷缺血的负面影响较小，从而改善了移植后的即时功能恢复，表明 HPG 是一种有前途的供体肾脏保存胶体。