Phytocompounds curcumin, quercetin, indole-3-carbinol, and resveratrol modulate lactate–pyruvate level along with cytotoxic activity in HeLa cervical cancer cells,Biotechnology and Applied Biochemistry

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Phytocompounds curcumin, quercetin, indole-3-carbinol, and resveratrol modulate lactate–pyruvate level along with cytotoxic activity in HeLa cervical cancer cells
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-10-25 , DOI: 10.1002/bab.2061
Sarita Pani ₁ , Amrita Sahoo ₁ , Abhilipsa Patra ₁ , Priya Ranjan Debata ₁

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Cancer cells meet their energy need by predominantly increased uptake of glucose, high rate of glycolysis, and increased production of lactate even in the presence of adequate oxygen. This process was proposed by Otto Warburg and named after him as the Warburg effect. The development of drugs that target glucose intake and aerobic glycolysis or lactic acid secretion of cancer cells is a newer approach for drug discovery. We have tested five purified plants-derived compounds such as curcumin, quercetin, ellagic acid, resveratrol, and indole-3-carbinol in HeLa cells for cytotoxicity, inhibition of metastasis, and modulation of lactate–pyruvate metabolism. Standard biochemical methods were used for glucose, lactic acid, and pyruvic acid measurement. The cell viability was determined by MTT assay. Cell migration was checked by wound healing assay. A dose-dependent cytotoxic effect and inhibition of cell migration were observed in all the tested compounds. A decrease in the lactate and increase in pyruvate level was observed in all the tested compounds except ellagic acid. Our finding suggests that tested phytocompounds are associated with the metabolic reprogramming of cancer cells and execute the cytotoxic effect. These compounds could be used for cancer prevention and therapy.

中文翻译：

植物化合物姜黄素、槲皮素、吲哚-3-甲醇和白藜芦醇可调节 HeLa 宫颈癌细胞中的乳酸-丙酮酸水平以及细胞毒活性

癌细胞主要通过增加葡萄糖摄取、高糖酵解速率和增加乳酸产量来满足其能量需求，即使在有足够氧气的情况下也是如此。这个过程是由 Otto Warburg 提出的，并以他的名字命名为 Warburg 效应。开发靶向葡萄糖摄入和有氧糖酵解或癌细胞乳酸分泌的药物是一种新的药物发现方法。我们已经在 HeLa 细胞中测试了五种纯化的植物衍生化合物，如姜黄素、槲皮素、鞣花酸、白藜芦醇和吲哚-3-甲醇，用于细胞毒性、抑制转移和调节乳酸-丙酮酸代谢。标准生化方法用于葡萄糖、乳酸和丙酮酸测量。通过MTT测定确定细胞活力。通过伤口愈合测定检查细胞迁移。在所有测试的化合物中都观察到了剂量依赖性的细胞毒性作用和细胞迁移的抑制作用。在除鞣花酸之外的所有测试化合物中都观察到乳酸减少和丙酮酸水平增加。我们的发现表明，经过测试的植物化合物与癌细胞的代谢重编程有关，并具有细胞毒性作用。这些化合物可用于癌症预防和治疗。

更新日期：2020-10-25

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