Bovine Lactoperoxidase (LP), a minor whey protein, is used as an antimicrobial in cosmetic, food, and pharmaceutical preparations. Industries are in pursuit of reliable, cheap, and scalable purification methods as the conventional techniques for LP purification like chromatography and membrane separation suffer from several drawbacks. The present work investigates the selective reverse micellar extraction of LP using the reverse micellar system formed by mixing food grade nonionic (Tween, Span, and Triton series) and ionic (AOT) surfactant blends. The analysis of LP extraction efficiency was performed by varying the concentration of nonionic surfactants with a constant AOT concentration of 100 mM and the initial pH of the system. Complete LP solubilization was achieved with reverse micelles formed by 100 mM AOT and 20 mM Tween 80 at pH 8. It was found that the extraction efficiency was dependent on the chain length or the number of ethylene oxide units in the Triton surfactant tail and the carbon–carbon double bond in Tween 80 tail, that is, on oleic acid. Span series however showed poor extraction in the organic phase substantiating the lesser water content. The forward extracted LP was successfully back‐extracted into a fresh aqueous phase containing 1 M KCl at pH 10.5. The aqueous phase (whey) from the forward transfer can be further used to fractionate other whey proteins.

中文翻译：

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用于从乳清中反胶束萃取乳过氧化物酶的离子和非离子表面活性剂混合物的分析

牛乳过氧化物酶（LP）是一种次要的乳清蛋白，在化妆品，食品和药物制剂中用作抗菌剂。工业上正在寻求可靠，廉价和可扩展的纯化方法，因为用于LP纯化的常规技术，例如色谱法和膜分离法具有若干缺点。本工作研究了通过将食品级非离子（吐温，司盘和特里顿系列）和离子（AOT）表面活性剂混合物混合而形成的反胶束系统，对LP进行选择性胶束萃取。通过以恒定的AOT浓度100 mM和系统的初始pH改变非离子表面活性剂的浓度来进行LP萃取效率的分析。用100 mM AOT和20 mM Tween 80在pH 8形成的反胶束可实现LP的完全溶解。发现萃取效率取决于链长或Triton表面活性剂尾部中环氧乙烷单元的数量以及Tween 80尾部中的碳-碳双键，即油酸。但是，Span系列显示出有机相中的萃取不良，这证明了水分含量较低。成功提取正向提取的LP，成功地将其反萃取到pH 10.5的含有1 M KCl的新鲜水相中。来自正向转移的水相（乳清）可以进一步用于分离其他乳清蛋白。但是，Span系列显示出有机相中的萃取不良，这证明了水分含量较低。成功提取正向提取的LP，成功地将其反萃取到pH 10.5的含有1 M KCl的新鲜水相中。来自正向转移的水相（乳清）可以进一步用于分离其他乳清蛋白。但是，Span系列显示出有机相中的萃取不良，这证明了水分含量较低。成功提取正向提取的LP，成功地将其反萃取到pH 10.5的含有1 M KCl的新鲜水相中。来自正向转移的水相（乳清）可以进一步用于分离其他乳清蛋白。