In this study, the effect of spirulina meal (Spirulina platensis) as a feed additive on growth and physiological response of Oscar fish,Astronotus ocellatus, was assessed using four diets including control without spirulina supplementation, 13.75 g/kg spirulina (SP1), 27.50 g/kg spirulina (SP2) and 55 g/kg spirulina (SP3). One hundred and twenty fish (10.41 ± 0.25 g) were disturbed to twelve aquariums and fed to apparent satiation three times a day for 8 weeks. The result showed that the SP3 treatment had statistically better growth performance, feeding parameters, total protease activity, total protein and lipid contents than the control. Moreover, haemoglobin, haematocrit, mean erythrocyte haemoglobin and mean erythrocyte haemoglobin concentrations were improved considerably in the SP3 treatment compared with the control. The SP3 treatment showed higher total protein and albumin levels, but lower triglycerides, cholesterol levels and liver enzyme activities than the control. Also, serum complements (C3, C4), lysozyme activity and total carotenoid contents in fish skin significantly increased in the SP3 treatment compared with the control. The results of this study suggest that spirulina powder at the level of 55 g/kg of the diet can be used as a functional natural feed additive to improve the performance of Oscar fish.

中文翻译：

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螺旋藻粉（Spirulina platensis）作为膳食添加剂对奥斯卡鱼，星状天牛的影响：评估生长性能，身体成分，消化酶活性，免疫生化参数，血液指标和总色素沉着

在这项研究中，螺旋藻粉（Spirulina platensis）作为饲料添加剂对Oscar鱼Astronotus ocellatus的生长和生理响应的影响，使用包括不添加螺旋藻的对照，13.75 g / kg螺旋藻（SP1），27.50 g / kg螺旋藻（SP2）和55 g / kg螺旋藻（SP3）在内的四种饮食来评估。一百二十条鱼（10.41±0.25克）被打扰到十二个水族箱中，并且每天喂饱三顿，连续8周。结果表明，SP3处理比对照具有更好的生长性能，饲喂参数，总蛋白酶活性，总蛋白质和脂质含量。而且，与对照相比，SP3处理中的血红蛋白，血细胞比容，平均红细胞血红蛋白和平均红细胞血红蛋白浓度显着提高。与对照组相比，SP3处理显示出更高的总蛋白和白蛋白水平，但甘油三酸酯，胆固醇水平和肝酶活性较低。也，与对照组相比，SP3处理的鱼皮中的血清补体（C3，C4），溶菌酶活性和总类胡萝卜素含量显着增加。这项研究的结果表明，可以将55 g / kg日粮中的螺旋藻粉用作功能性天然饲料添加剂，以改善奥斯卡鱼的性能。