Relative Protein Abundances and Biological Ageing in Whole Skeletal Elements,Journal of Proteome Research

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Relative Protein Abundances and Biological Ageing in Whole Skeletal Elements
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-10-22 , DOI: 10.1021/acs.jproteome.0c00555
Elizabeth Johnston ₁ , Michael Buckley ₁

Affiliation

Establishing biological age is an integral part of forensic investigations, currently achieved through morphological methods with varying degrees of accuracy. Furthermore, biological ageing is much easier in juveniles than in adults, at which point traditional ageing methods struggle. Therefore, biomolecular approaches are considered of great interest, with several protein markers already recognized for their potential forensic significance. However, previous studies have typically relied on subsampling different parts of skeletal elements. Here, we attempt to evaluate the proteome of complete elements using a rat model. In the analysis of specimens spanning beyond adulthood (1 week to 1.5 years), we observed 729 unique proteins across 33 samples (three for each sex for each of the five (female) or six (male)), five of which represent newly identified proteins in relation to age estimation: vimentin, osteopontin, matrilin-1, apolipoprotein A-I, and prothrombin. Most of these follow the trend of decreasing abundance through age, with the exception of prothrombin that increases. We consider the combined use of these relative abundances, along with those of previously noted fetuin-A, biglycan, albumin, and chromogranin-A signatures, as being of potential value to the development of an age estimation tool worthy of further evaluation in forensic contexts.

中文翻译：

整个骨骼元素中的相对蛋白质丰度和生物衰老

建立生物年龄是法医调查的组成部分，目前，法医调查是通过形态学方法以不同程度的准确性实现的。此外，与成年人相比，青少年的生物衰老要容易得多，这时传统的衰老方法难以解决。因此，生物分子方法被认为是非常令人感兴趣的，几种蛋白质标记物已经被认为具有潜在的法医学意义。但是，以前的研究通常依赖于对骨骼元素的不同部分进行二次采样。在这里，我们尝试使用大鼠模型评估完整元件的蛋白质组。在对超过成年期（1周至1.5岁）的标本进行分析时，我们在33个样本中观察到729种独特的蛋白质（五个（女性）或六个（男性）中的每个性别为三个），其中五种代表与年龄估计有关的新发现蛋白：波形蛋白，骨桥蛋白，matrilin-1，载脂蛋白AI和凝血酶原。除凝血酶原增加外，大多数都遵循随着年龄增长而减少的趋势。我们认为这些相对丰度的结合使用以及先前提到的胎球蛋白-A，双糖链蛋白，白蛋白和嗜铬粒蛋白-A标记的结合使用，对于开发值得在法医背景下进一步评估的年龄估算工具具有潜在价值。。

更新日期：2020-10-22

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