QuantPipe: A User-Friendly Pipeline Software Tool for DIA Data Analysis Based on the OpenSWATH-PyProphet-TRIC Workflow,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

QuantPipe: A User-Friendly Pipeline Software Tool for DIA Data Analysis Based on the OpenSWATH-PyProphet-TRIC Workflow
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-10-22 , DOI: 10.1021/acs.jproteome.0c00704
Dazheng Wang ₁ , Guohong Gan ₁ , Xi Chen _{2,

3} , Chuan-Qi Zhong ₁

Affiliation

Targeted analysis of data-independent acquisition (DIA) mass spectrometry data requires elegant software tools and strict statistical control. OpenSWATH-PyProphet-TRIC is a widely used DIA data analysis workflow. The OpenSWATH-PyProphet-TRIC workflow is typically executed by running command lines. Here, we present QuantPipe, which is a graphic interface software tool based on the OpenSWATH-PyProphet-TRIC workflow. In addition to OpenSWATH-PyProphet-TRIC functions, QuantPipe can convert the spectral library to the assay library and output peptides and protein intensities. We demonstrated that QuantPipe can be used to analyze SWATH-MS data from TripleTOF 5600 and TripleTOF 6600, phospho-SWATH-MS data, DIA data from Orbitrap instrument, and diaPASEF data from TimsTOF Pro instrument. The executable files, user manual, and source code of QuantPipe are freely available at https://github.com/tachengxmu/QuantPipe/releases.

中文翻译：

QuantPipe：基于OpenSWATH-PyProphet-TRIC工作流程的用于DIA数据分析的用户友好管道软件工具

数据独立采集（DIA）质谱数据的目标分析需要精美的软件工具和严格的统计控制。OpenSWATH-PyProphet-TRIC是广泛使用的DIA数据分析工作流。OpenSWATH-PyProphet-TRIC工作流程通常通过运行命令行来执行。在这里，我们介绍QuantPipe，这是一个基于OpenSWATH-PyProphet-TRIC工作流程的图形界面软件工具。除了OpenSWATH-PyProphet-TRIC功能外，QuantPipe还可以将光谱库转换为化验库，并输出肽段和蛋白质强度。我们证明了QuantPipe可用于分析TripleTOF 5600和TripleTOF 6600的SWATH-MS数据，phospho-SWATH-MS数据，Orbitrap仪器的DIA数据以及TimsTOF Pro仪器的diaPASEF数据。可执行文件，用户手册，

更新日期：2020-10-22

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11