Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy,Analytical Chemistry

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Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-22 , DOI: 10.1021/acs.analchem.0c02489
Jiabao Xu _{1,

2} , Xiaojie Li _{1,

3,

4} , Zhongyue Guo ₁ , Wei E Huang ₂ , Ji-Xin Cheng ₁

Affiliation

We report rapid and sensitive phenotyping of bacterial response to antibiotic treatment at single-cell resolution by a Raman-integrated optical mid-infrared photothermal (MIP) microscope. The MIP microscope successfully detected biochemical changes of bacteria in specific to the acting mechanism of erythromycin with 1 h incubation. Compared to Raman spectroscopy, MIP spectroscopy showed a much larger signal-to-noise ratio at the fingerprint region at an acquisition speed as fast as 1 s per spectrum. The high sensitivity of MIP enabled detection of metabolic changes at antibiotic concentrations below minimum inhibitory concentration (MIC). Meanwhile, the single-cell resolution of the technique allowed observation of heteroresistance within one bacterial population, which is of great clinical relevance. This study showcases characterizing antibiotic response as one of the many possibilities of applying MIP microscopy to single-cell biology.

中文翻译：

通过拉曼集成中红外光热显微镜指纹识别细菌对红霉素的代谢反应

我们报告了通过拉曼集成光学中红外光热 (MIP) 显微镜以单细胞分辨率快速、灵敏地分析细菌对抗生素治疗反应的表型。MIP显微镜在孵育1 h后成功检测到细菌针对红霉素作用机制的生化变化。与拉曼光谱相比，MIP 光谱在指纹区域显示出更大的信噪比，采集速度快至每个光谱 1 秒。MIP 的高灵敏度使得能够在低于最低抑制浓度 (MIC) 的抗生素浓度下检测代谢变化。同时，该技术的单细胞分辨率允许观察一个细菌群体内的异质抗性，这具有很大的临床意义。这项研究展示了将抗生素反应表征为将 MIP 显微镜应用于单细胞生物学的众多可能性之一。

更新日期：2020-11-03

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