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Visualization of ONOO– and Viscosity in Drug-Induced Hepatotoxicity with Different Fluorescence Signals by a Sensitive Fluorescent Probe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-22 , DOI: 10.1021/acs.analchem.0c03199 Yingzhen Deng 1 , Guoqiang Feng 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-22 , DOI: 10.1021/acs.analchem.0c03199 Yingzhen Deng 1 , Guoqiang Feng 1
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Drug-induced liver injury (DILI) is considered gradually as a serious public health issue, and hepatotoxicity has been regarded as the main clinical problem caused by it. We suspected that both the intracellular viscosity and peroxynitrite (ONOO–) levels in drug-induced hepatotoxicity tissue are higher than those in a healthy liver. For this reason, we have presented a fluorescent probe VO for multichannel imaging viscosity and ONOO– simultaneously. Experimental results showed that VO has satisfactory detection performance for both viscosity and ONOO–, and based on the advantages of its lower cytotoxicity and pH-stabilities, VO was successfully employed to image viscosity and ONOO– in living cells and animals. More importantly, we use the probe to successfully showcase drug-induced hepatotoxicity by imaging viscosity and ONOO– induced by acetaminophen (APAP). All the results indicate that VO has great potential for the detection of viscosity and ONOO– and to assay drug-induced hepatotoxicity. Overall, this work offers a new detection tool/method for a deeper understanding of drug-induced organism injury.
中文翻译：
ONOO的可视化-通过一个敏感的荧光探针和黏度的药物诱导的肝毒性与不同的荧光信号
药物性肝损伤（DILI）逐渐被认为是严重的公共卫生问题，并且肝毒性已被视为由其引起的主要临床问题。我们怀疑药物诱导的肝毒性组织中的细胞内粘度和过氧亚硝酸盐（ONOO –）水平都高于健康肝脏中的水平。出于这个原因，我们已经提出的荧光探针VO用于多声道成像粘度和ONOO -同时。实验结果表明，VO具有用于粘度和ONOO令人满意的检测性能- ，和基于其较低的细胞毒性和pH稳定性的优点，VO被成功地用于图像粘度和ONOO–在活细胞和动物中。更重要的是，我们通过成像粘度和ONOO使用探头成功地展示药物引起的肝毒性-对乙酰氨基酚（APAP）诱导。以上结果表明，VO有用于检测粘度和ONOO的巨大潜力-并测定药物引起的肝毒性。总的来说，这项工作提供了一种新的检测工具/方法，可以更深入地了解药物引起的生物体损伤。
更新日期：2020-11-03
中文翻译：
ONOO的可视化-通过一个敏感的荧光探针和黏度的药物诱导的肝毒性与不同的荧光信号
药物性肝损伤（DILI）逐渐被认为是严重的公共卫生问题，并且肝毒性已被视为由其引起的主要临床问题。我们怀疑药物诱导的肝毒性组织中的细胞内粘度和过氧亚硝酸盐（ONOO –）水平都高于健康肝脏中的水平。出于这个原因，我们已经提出的荧光探针VO用于多声道成像粘度和ONOO -同时。实验结果表明，VO具有用于粘度和ONOO令人满意的检测性能- ，和基于其较低的细胞毒性和pH稳定性的优点，VO被成功地用于图像粘度和ONOO–在活细胞和动物中。更重要的是，我们通过成像粘度和ONOO使用探头成功地展示药物引起的肝毒性-对乙酰氨基酚（APAP）诱导。以上结果表明，VO有用于检测粘度和ONOO的巨大潜力-并测定药物引起的肝毒性。总的来说，这项工作提供了一种新的检测工具/方法，可以更深入地了解药物引起的生物体损伤。
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