Immobilization of the extracellular recombinant Lucky9 xylanase from Bacillus subtilis enhances activity at high temperature and pH,FEBS Open Bio

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Immobilization of the extracellular recombinant Lucky9 xylanase from Bacillus subtilis enhances activity at high temperature and pH
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-10-22 , DOI: 10.1002/2211-5463.13010
Sai-Sai Ding ₁ , Jin-Peng Zhu ₁ , Yang Wang ₁ , Bin Wu ₂ , Zongpei Zhao ₁

Affiliation

In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco‐friendly process called biobleaching, which can help minimize the usage of chlorine in the delignification process. However, a bottleneck in the adoption of biobleaching is the cost of xylanase and the requirement that xylanase be active and stable at extreme conditions. Here, we investigated whether using sodium alginate beads to immobilize an extracellular xylanase from Bacillus subtilis (Lucky9) can reduce the potential cost of enzyme usage. The optimal pH and the activity of the immobilized enzyme were increased at optimal temperature compared with the free enzyme. In addition, immobilized xylanase was shown to be more stable than free xylanase. The results of this study suggest that the immobilized xylanase has potential applications in the biobleaching industry.

中文翻译：

来自枯草芽孢杆菌的细胞外重组 Lucky9 木聚糖酶的固定化增强了高温和 pH 值下的活性

在造纸工业中，氯通常用于处理纸浆以进行漂白。制浆后，纤维中存在大量木聚糖。木聚糖酶可用于在称为生物漂白的生态友好过程中降解木聚糖，这有助于最大限度地减少脱木素过程中氯的使用。然而，采用生物漂白的一个瓶颈是木聚糖酶的成本以及木聚糖酶在极端条件下具有活性和稳定性的要求。在这里，我们研究了是否使用海藻酸钠珠来固定来自枯草芽孢杆菌的细胞外木聚糖酶(Lucky9) 可以降低酶使用的潜在成本。与游离酶相比，在最适温度下固定化酶的最适 pH 值和活性有所提高。此外，固定化木聚糖酶比游离木聚糖酶更稳定。本研究结果表明，固定化木聚糖酶在生物漂白行业具有潜在的应用价值。

更新日期：2020-12-03

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