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Insight into watery saliva proteomes of the grain aphid, Sitobion avenae
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-10-20 , DOI: 10.1002/arch.21752
Yong Zhang 1 , Yu Fu 1 , Frédéric Francis 2 , Xiaobei Liu 1 , Julian Chen 1
Affiliation  

The grain aphid, Sitobion avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. avenae. Here, the watery saliva proteins from S. avenae were collected in an artificial diet and identified using a liquid chromatography–mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. avenae saliva and speculated to play a significant role in aphid–plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.

中文翻译:

洞悉谷物蚜虫(Sitobion avenae)的含水唾液蛋白质组

谷物蚜虫,Sitobion avenae,是一种在世界范围内具有重要经济意义的谷物害虫。蚜虫唾液在蚜虫与其寄主植物之间的相互作用中起着至关重要的作用。然而,关于在葡萄球菌的唾液中发现的蛋白质的信息有限。在这里,在人工饮食中收集了来自链球菌的含水唾液蛋白,并使用液相色谱-质谱/质谱分析法对其进行了鉴定。鉴定出114种蛋白质唾液,包括几种酶,结合蛋白和推定的效应子,以及其他功能未知的蛋白。与来自其他九个蚜虫唾液蛋白的比较,从唾液蛋白的最显着的特征麦长管蚜是的蛋白质的功能的不同的图案。其他蚜虫物种分泌的几种直系同源蛋白质,例如葡萄糖脱氢酶,延伸因子和效应物C002,也可以在avenger S.唾液中检测到,并推测在蚜虫-植物相互作用中起重要作用。这些结果为进一步了解蚜虫和谷物与植物之间的相互作用提供了分子基础。
更新日期:2020-12-26
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